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酿酒酵母野生型葡萄酒菌株的蛋白质组学特征分析

Proteomic characterization of a wild-type wine strain of Saccharomyces cerevisiae.

作者信息

Trabalzini Lorenza, Paffetti Alessandro, Ferro Elisa, Scaloni Andrea, Talamo Fabio, Millucci Lia, Martelli Paola, Santucci Annalisa

机构信息

Dipartimento di Biologia Molecolare, Universita' degli Studi di Siena, via Fiorentina 1, 53100 Siena, Italy.

出版信息

Ital J Biochem. 2003 Dec;52(4):145-53.

Abstract

Saccharomyces cerevisiae is the optimal eukaryotic model system to study mammalian biological responses. At the same time Saccharomyces cerevisiae is also widely utilized as a biotechnological tool in the food industry. Enological Saccharomyces cerevisiae strains have been so far routinely analyzed for their microbiological aspects. Nevertheless, wine yeasts are gaining an increasing interest in the last years since they strongly affect both the vinification process and the organoleptic properties of the final product wine. The protein repertoire is responsible of such features and, consequently, 2D-PAGE can be an useful tool to evaluate and select optimal wine yeast strains. We present here the first proteomic map of a wild-type wine Saccharomyces cerevisiae strain selected for the guided fermentation of very high quality wines.

摘要

酿酒酵母是研究哺乳动物生物学反应的最佳真核模式系统。同时,酿酒酵母在食品工业中也被广泛用作生物技术工具。到目前为止,已对酿酒工业用酿酒酵母菌株的微生物学方面进行了常规分析。然而,葡萄酒酵母在过去几年中越来越受到关注,因为它们对酿酒过程和最终产品葡萄酒的感官特性都有很大影响。蛋白质组决定了这些特性,因此,二维聚丙烯酰胺凝胶电泳(2D-PAGE)可以作为评估和选择最佳葡萄酒酵母菌株的有用工具。我们在此展示了一种野生型葡萄酒酿酒酵母菌株的首张蛋白质组图谱,该菌株被选用于高品质葡萄酒的定向发酵。

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