Lefièvre L, Conner S J, Salpekar A, Olufowobi O, Ashton P, Pavlovic B, Lenton W, Afnan M, Brewis I A, Monk M, Hughes D C, Barratt C L R
University of Birmingham, B15 2TG, UK.
Hum Reprod. 2004 Jul;19(7):1580-6. doi: 10.1093/humrep/deh301. Epub 2004 May 13.
The zona pellucida (ZP) is an extracellular glycoprotein matrix which surrounds all mammalian oocytes. Recent data have shown the presence of four human zona genes (ZP1, ZP2, ZP3 and ZPB). The aim of the study was to determine if all four ZP proteins are expressed and present in the human.
cDNA derived from human oocytes were used to amplify by PCR the four ZP genes. In addition, isolated native human ZP were heat-solubilized, trypsin-digested and subjected to tandem mass spectrometry (MS/MS).
All four genes were expressed and the respective proteins present in the human ZP. Moreover, a bioinformatics approach showed that the mouse ZPB gene, although present, is likely to encode a non-functional protein.
Four ZP genes are expressed in human oocytes (ZP1, ZP2, ZP3 and ZPB) and preliminary data show that the four corresponding ZP proteins are present in the human ZP. Therefore, this is a fundamental difference with the mouse model
透明带（ZP）是一种围绕所有哺乳动物卵母细胞的细胞外糖蛋白基质。最近的数据显示存在四种人类透明带基因（ZP1、ZP2、ZP3和ZPB）。本研究的目的是确定这四种ZP蛋白是否在人类中表达并存在。
用人卵母细胞衍生的cDNA通过PCR扩增四种ZP基因。此外，将分离的天然人类ZP进行热溶解、胰蛋白酶消化并进行串联质谱分析（MS/MS）。
所有四个基因均表达，且相应蛋白存在于人类ZP中。此外，一种生物信息学方法表明，小鼠ZPB基因虽然存在，但可能编码一种无功能的蛋白。
四种ZP基因在人类卵母细胞中表达（ZP1、ZP2、ZP3和ZPB），初步数据表明四种相应的ZP蛋白存在于人类ZP中。因此，这与小鼠模型存在根本差异。
Zotero 插件