Krivoĭ I I, Drabkina T M, Dobretsov M G, Vasil'ev A N, Kravtsova V V, Eaton M J, Skachkov S N, Mandel F
St. Petersburg State University, 199034, St. Petersburg, University Emb., 7/9, Russia.
Ross Fiziol Zh Im I M Sechenova. 2004 Jan;90(1):59-72.
Acetylcholine (ACh) hyperpolarized the rat diaphragm muscle fibers by 4.5 +/- 0.8 mV (K0.5 = = 36 +/- 6 nmol/l). The AC-induced hyperpolarization was blocked by d-tubocurarine and ouabain in nanomolar concentrations. This effect of ACh was not observed in cultured C2C12 muscle cells and in Xenopus oocytes with expressed embryonic mouse muscle nicotinic acetylcholine receptors (nAChR) or with neuronal alpha 4 beta 2 nAChR. In membrane preparations from the Torpedo californica electric organ, containing both nAChR and Na, K-ATPase, 10 nmol/l ouabain modulated the binding kinetics of the cholinergic ligand dansyl-C6-choline to the nAChR. These results suggest that in-sensitive alpha 2 isoform) and nAChR in a state with high affinity to Ach and d-tubocurarine may form a functional complex in which binding of ACh to nAchR is coupled to activation of the Na, K-ATPase.
乙酰胆碱（ACh）使大鼠膈肌纤维超极化4.5±0.8 mV（半数效应浓度[K0.5] = 36±6 nmol/L）。纳摩尔浓度的筒箭毒碱和哇巴因可阻断交流电诱导的超极化。在培养的C2C12肌肉细胞以及表达胚胎小鼠肌肉烟碱型乙酰胆碱受体（nAChR）或神经元α4β2 nAChR的非洲爪蟾卵母细胞中未观察到ACh的这种作用。在含有nAChR和钠钾ATP酶的加州电鳐电器官的膜制剂中，10 nmol/L哇巴因调节了胆碱能配体丹磺酰基 - C6 - 胆碱与nAChR的结合动力学。这些结果表明，不敏感的α2亚型和对乙酰胆碱和筒箭毒碱具有高亲和力状态的nAChR可能形成一种功能复合物，其中ACh与nAchR的结合与钠钾ATP酶的激活相偶联。
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