Finnie C, Maeda K, ØStergaard O, Bak-Jensen K S, Larsen J, Svensson B
Department of Chemistry, Carlsberg Laboratory, Gamle Carlsberg Vej 10, DK-2500 Valby, Denmark.
Biochem Soc Trans. 2004 Jun;32(Pt3):517-9. doi: 10.1042/BST0320517.
Analysis of the water-soluble barley seed proteome has led to the identification of proteins by MS in the major spots on two-dimensional gels covering the pI ranges 4-7 and 6-11. This provides the basis for in-depth studies of proteome changes during seed development and germination, tissue-specific proteomes, cultivar differences related to quality parameters, analysis of the genetic basis for spot variations and targeted investigations of specific proteins.
对水溶性大麦种子蛋白质组的分析已通过质谱法鉴定出二维凝胶上主要斑点中的蛋白质，这些二维凝胶覆盖了4 - 7和6 - 11的等电点范围。这为深入研究种子发育和萌发过程中的蛋白质组变化、组织特异性蛋白质组、与品质参数相关的品种差异、斑点变异的遗传基础分析以及特定蛋白质的靶向研究提供了基础。
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