嗅觉感觉图谱的建立和维持需要自发神经活动。

Spontaneous neural activity is required for the establishment and maintenance of the olfactory sensory map.

作者信息

Yu C Ron, Power Jennifer, Barnea Gilad, O'Donnell Sean, Brown Hannah E V, Osborne Joseph, Axel Richard, Gogos Joseph A

机构信息

Center for Neurobiology and Behavior, Columbia University, New York, NY 10032, USA.

出版信息

Neuron. 2004 May 27;42(4):553-66. doi: 10.1016/s0896-6273(04)00224-7.

DOI:10.1016/s0896-6273(04)00224-7

PMID:15157418

Abstract

We have developed a genetic approach to examine the role of spontaneous activity and synaptic release in the establishment and maintenance of an olfactory sensory map. Conditional expression of tetanus toxin light chain, a molecule that inhibits synaptic release, does not perturb targeting during development, but neurons that express this molecule in a competitive environment fail to maintain appropriate synaptic connections and disappear. Overexpression of the inward rectifying potassium channel, Kir2.1, diminishes the excitability of sensory neurons and more severely disrupts the formation of an olfactory map. These studies suggest that spontaneous neural activity is required for the establishment and maintenance of the precise connectivity inherent in an olfactory sensory map.

摘要

我们已经开发出一种遗传学方法，以研究自发活动和突触释放在嗅觉感觉图谱的建立和维持中的作用。破伤风毒素轻链是一种抑制突触释放的分子，其条件性表达在发育过程中不会干扰靶向，但在竞争环境中表达该分子的神经元无法维持适当的突触连接并消失。内向整流钾通道Kir2.1的过表达会降低感觉神经元的兴奋性，并更严重地破坏嗅觉图谱的形成。这些研究表明，自发神经活动是建立和维持嗅觉感觉图谱中固有精确连接所必需的。