三种古菌糖ABC转运蛋白ATP酶亚基的分子进化

Molecular evolution of the ATPase subunit of three archaeal sugar ABC transporters.

作者信息

Imamura Hiromi, Jeon Beon-Sam, Wakagi Takayoshi

机构信息

Department of Biotechnology, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.

出版信息

Biochem Biophys Res Commun. 2004 Jun 18;319(1):230-4. doi: 10.1016/j.bbrc.2004.04.174.

DOI:10.1016/j.bbrc.2004.04.174

PMID:15158466

Abstract

The sequence of genes encoding extracellular amylopullulanase and putative maltodextrin ATP binding cassette (ABC) transporter of a hyperthermophilic archaeon, Thermococcus litoralis, was determined. The mdxK gene, which encodes an ATPase subunit of the putative maltodextrin ABC transporter, has extraordinarily high similarity with the malK gene, which encodes an ATPase subunit of trehalose/maltose ABC transporter of the same organism. DNA sequence comparison revealed that the malK gene was generated through the duplication of the mdxK gene before lateral gene transfer of the mal gene cluster from T. litoralis to Pyrococcus furiosus.

摘要

测定了嗜热古菌嗜热栖热菌编码细胞外支链淀粉酶和假定的麦芽糊精ATP结合盒（ABC）转运蛋白的基因序列。编码假定麦芽糊精ABC转运蛋白ATP酶亚基的mdxK基因，与编码同一生物体海藻糖/麦芽糖ABC转运蛋白ATP酶亚基的malK基因具有极高的相似性。DNA序列比较表明，malK基因是在mal基因簇从嗜热栖热菌侧向基因转移到激烈火球菌之前，通过mdxK基因的复制产生的。