Martínez María A, Lajas Ana I, Yago María D, Redondo Pedro C, Granados María P, González Antonio, Rosado Juan A, Martínez-Victoria Emilio, Mañas Mariano, Pariente José A
Institute of Nutrition and Food Technology, Department of Physiology, University of Granada, Granada, Spain.
Nutrition. 2004 Jun;20(6):536-41. doi: 10.1016/j.nut.2004.03.018.
We evaluated the long-term effects of a fat-enriched diet (virgin olive oil) on calcium mobilization and amylase secretion induced by cholecystokinin-octapeptide (CCK-8) in rat pancreatic acinar cells. Olive oil is a major component of the Mediterranean diet, and its role in human health is actively being debated.
Weaning male Wistar rats (21 d old) were assigned to one of two experimental groups and fed for 8 wk with a commercial chow (control group) or an experimental diet (olive group) containing 100 g/kg of virgin olive oil as dietary fat. Intracellular free calcium Ca(2+) levels were determined by loading the pancreatic cells with the fluorescent ratio-metric calcium indicator Fura-2 on an inverted fluorescent microscope. For measurement of amylase secretion, cells were incubated with the appropriate secretagogue for 30 min, and amylase activities in the supernatant were determined by the Phadebas blue starch method. Analysis of variance was used to test differences between groups.
Compared with the control group, the CCK-8-induced increase in Ca(2+) occurred in cells from rats in the olive group (P < 0.05). This stimulatory effect of dietary virgin olive oil was observed in calcium oscillations and large Ca(2+) transients induced by low (20 pM/L) and high (10 nM/L) concentrations of CCK-8, respectively. In addition to the effects of dietary virgin olive oil on calcium mobilization, it increased (P < 0.05) amylase secretion in response to CCK-8. Olive oil treatment did not significantly alter resting Ca(2+) or amylase release values compared with the control group. Similar results were obtained when pancreatic acinar cells were stimulated with a high concentration of acetylcholine (10 microM/L).
The present results demonstrate that a diet supplemented with virgin olive oil can modify pancreatic cell function as assessed by Ca(2+) mobilization and amylase release evoked by secretagogues in rat pancreatic acinar cells. A role for fatty acids in calcium signaling is suggested.
我们评估了富含脂肪的饮食（初榨橄榄油）对大鼠胰腺腺泡细胞中胆囊收缩素八肽（CCK-8）诱导的钙动员和淀粉酶分泌的长期影响。橄榄油是地中海饮食的主要成分，其在人类健康中的作用正受到积极讨论。
将断乳雄性Wistar大鼠（21日龄）分为两个实验组之一，分别用商业饲料（对照组）或含100 g/kg初榨橄榄油作为膳食脂肪的实验性饮食（橄榄油组）喂养8周。通过在倒置荧光显微镜上用荧光比率测量钙指示剂Fura-2加载胰腺细胞来测定细胞内游离钙Ca(2+)水平。为了测量淀粉酶分泌，将细胞与适当的促分泌剂孵育30分钟，并用Phadebas蓝淀粉法测定上清液中的淀粉酶活性。采用方差分析来检验组间差异。
与对照组相比，橄榄油组大鼠细胞中CCK-8诱导的Ca(2+)增加（P < 0.05）。在分别由低浓度（20 pM/L）和高浓度（10 nM/L）CCK-8诱导的钙振荡和大的Ca(2+)瞬变中观察到了膳食初榨橄榄油的这种刺激作用。除了膳食初榨橄榄油对钙动员的影响外，它还增加了（P < 0.05）对CCK-8的淀粉酶分泌。与对照组相比，橄榄油处理未显著改变静息Ca(2+)或淀粉酶释放值。当用高浓度乙酰胆碱（10 microM/L）刺激胰腺腺泡细胞时，获得了类似的结果。
目前的结果表明，补充初榨橄榄油的饮食可以改变胰腺细胞功能，这通过大鼠胰腺腺泡细胞中促分泌剂诱发的Ca(2+)动员和淀粉酶释放来评估。提示脂肪酸在钙信号传导中起作用。
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