一种来自糙皮侧耳（平菇）的新型核糖核酸酶。

A new ribonuclease from the black oyster mushroom Pleurotus ostreatus.

作者信息

Wang H X, Ng T B

机构信息

Department of Microbiology, China Agricultural University, Beijing, and State Key Laboratory of Agrobiotechnology, Beijing, China.

出版信息

Peptides. 2004 Apr;25(4):685-7. doi: 10.1016/j.peptides.2004.01.017.

DOI:10.1016/j.peptides.2004.01.017

PMID:15165725

Abstract

A ribonuclease that is co-specific for poly C and poly U has been isolated from the fruiting bodies of the black oyster mushroom. The enzyme possesses a molecular mass of 14 kDa, and is unadsorbed on DEAE-cellulose and adsorbed on Affi-gel blue gel and CM-cellulose. A pH of 7 is required for the enzyme to exhibit maximal activity. The activity of the enzyme does not vary appreciably over the temperature range 30-60 degrees C, but drops when the temperature is reduced to 20 degrees C or raised to and above 70 degrees C. The ribonuclease does not exert any inhibitory activity toward HIV-1 reverse transcriptase.

摘要

一种对聚C和聚U具有共同特异性的核糖核酸酶已从黑平菇子实体中分离出来。该酶的分子量为14 kDa，不吸附于DEAE-纤维素，吸附于Affi-凝胶蓝凝胶和CM-纤维素。该酶需要pH 7才能表现出最大活性。在30-60摄氏度的温度范围内，该酶的活性变化不明显，但当温度降至20摄氏度或升至70摄氏度及以上时，活性会下降。该核糖核酸酶对HIV-1逆转录酶没有任何抑制活性。