reg Iα蛋白在人胃癌中的表达

Expression of reg I alpha protein in human gastric cancers.

作者信息

Fukui Hirokazu, Fujii Shigehiko, Takeda Jun, Kayahara Takahisa, Sekikawa Akira, Nanakin Apichart, Suzuki Katsumasa, Hisatsune Hiroshi, Seno Hiroshi, Sawada Mitsutaka, Fujimori Takahiro, Chiba Tsutomu

机构信息

Department of Gastroenterology and Hepatology, Kyoto University Graduate School of Medicine, Sakyo Kyoto, Japan.

出版信息

Digestion. 2004;69(3):177-84. doi: 10.1159/000078762. Epub 2004 May 27.

DOI:10.1159/000078762

PMID:15166487

Abstract

BACKGROUND/AIMS: Although regenerating gene(Reg) I alpha protein has a trophic effect on gastric epithelial cells, it is unclear whether Reg I alpha protein and its receptor are involved in gastric carcinogenesis. Therefore, we investigated the Reg I alpha protein expression in human gastric cancers and assessed its relationship to clinicopathological factors.

METHODS

Sixty-one gastric cancer specimens were examined, using immunohistochemistry, for Reg I alpha protein, p53, and proliferating cell nuclear antigen. The expression of both Reg I alpha and Reg receptor mRNA was examined in seven human gastric cancer cell lines (MKN1, MKN28, MKN45, MKN74, KATOIII, GCIY, and AGS) by reverse transcription-polymerase chain reaction and Northern blot analysis.

RESULTS

Twenty-three (37.7%) of the 61 gastric cancer tissues samples were positive for Reg I alpha protein. The Reg I alpha expression was significantly related to the presence of lymphatic invasion but not to tumor size, tumor stage, Lauren's classification, presence of venous invasion, lymph node metastases, or p53 overexpression. Gastric cancers positive for Reg I alpha protein showed a significantly higher proliferating cell nuclear antigen labeling index than negative ones. The expression of both Reg I alpha and Reg receptor mRNA was detected in all seven gastric cancer cell lines.

CONCLUSION

Reg I alpha protein may play a role in the development of gastric cancers.

摘要

背景/目的：尽管再生基因（Reg）Iα蛋白对胃上皮细胞具有营养作用，但Reg Iα蛋白及其受体是否参与胃癌发生尚不清楚。因此，我们研究了Reg Iα蛋白在人胃癌中的表达，并评估其与临床病理因素的关系。

方法

采用免疫组织化学方法检测61例胃癌标本中Reg Iα蛋白、p53和增殖细胞核抗原。通过逆转录-聚合酶链反应和Northern印迹分析检测7种人胃癌细胞系（MKN1、MKN28、MKN45、MKN74、KATOIII、GCIY和AGS）中Reg Iα和Reg受体mRNA的表达。

结果

61例胃癌组织样本中有23例（37.7%）Reg Iα蛋白呈阳性。Reg Iα表达与淋巴浸润显著相关，但与肿瘤大小、肿瘤分期、劳伦分类、静脉浸润、淋巴结转移或p53过表达无关。Reg Iα蛋白阳性的胃癌增殖细胞核抗原标记指数显著高于阴性者。在所有7种胃癌细胞系中均检测到Reg Iα和Reg受体mRNA的表达。