抗鼻病毒化合物2-呋喃基氯化汞的作用模式。

Mode of action of 2-furylmercury chloride, an anti-rhinovirus compound.

作者信息

Verheyden B, Andries K, Rombaut B

机构信息

Department of Microbiology and Hygiene, Vrije Universiteit Brussel, Laarbeeklaan 103, Brussels B-1090, Belgium.

出版信息

Antiviral Res. 2004 Mar;61(3):189-94. doi: 10.1016/j.antiviral.2003.10.004.

DOI:10.1016/j.antiviral.2003.10.004

PMID:15168800

Abstract

2-Furylmercury chloride (2-FMC), an organic mercury derivative, has been found to inhibit the replication of all tested human rhinovirus (HRV) serotypes belonging to the antiviral group B and a limited number of HRV serotypes belonging to the antiviral group A. The mechanism of action of 2-FMC was tested against HRV-2 (antiviral group B, minor receptor group), and compared with an antiviral compound for which the viral target was already determined (enviroxime). 2-FMC was found to bind reversibly to virus particles. However, time-dependent plaque reduction assays revealed that 2-FMC did not interfere with early events of HRV-2 replication. Using a quantitative RT-PCR ELISA assay, we were able to prove that 2-FMC inhibits the synthesis of viral RNA. However, the mode of action of 2-FMC is not identical to that of enviroxime, another inhibitor of viral RNA synthesis. Time-of-addition and time-of-withdrawal experiments demonstrated that 2-FMC acted during a broader time interval than enviroxime.

摘要

2-氯汞基呋喃（2-FMC）是一种有机汞衍生物，已发现它能抑制所有属于B组抗病毒型的测试人鼻病毒（HRV）血清型以及少数属于A组抗病毒型的HRV血清型的复制。针对HRV-2（B组抗病毒型，次要受体组）测试了2-FMC的作用机制，并与一种病毒靶点已确定的抗病毒化合物（恩韦肟）进行了比较。发现2-FMC与病毒颗粒可逆结合。然而，时间依赖性蚀斑减少试验表明，2-FMC不干扰HRV-2复制的早期事件。使用定量逆转录聚合酶链反应酶联免疫吸附测定法，我们能够证明2-FMC抑制病毒RNA的合成。然而，2-FMC的作用方式与另一种病毒RNA合成抑制剂恩韦肟不同。添加时间和去除时间实验表明，2-FMC的作用时间间隔比恩韦肟更宽。