[基于蛋白质盐和直链淀粉的手性固定相对盐酸西替利嗪对映体分离的研究]

[Study on the enantiomer separation of cetirizine dihydrochloride using proteinate- and amylose-based chiral stationary phase].

作者信息

Zhang Zhe-feng, Yang Geng-liang, Liang Gui-jian, Zhou Yu, Chen Yi

机构信息

College of Chemistry and Environmental Science, Hebei University, Baoding 071002, China.

出版信息

Yao Xue Xue Bao. 2004 Mar;39(3):204-7.

PMID:15171656

Abstract

AIM

To study the chromatographic behavior of cetirizine dihydrochloride on the proteinate- and amylose- based chiral stationary phases so as to optimizate the chromatographic condition of its enantiomers separation.

METHODS

When using amylose-based, alpha1-acid glycoprotein and ovomucoid protein chiral stationary phase, the mobile phase was hexane-isopropyl alcohol-alcohol-trifluoroacetic acid (430:45:25:1), acetonitrile-10 mmol x L(-1) phosphate buffer solution (adjusted to pH 7.0 with sodium hydroxide) (4:96) and acetonitrile-20 mmol x L(-1) KH, PO4 solution (adjusted to pH 7.0 with triethylamine) (12.7:87.3), respectively. The temperature of proteinate column was 25 degrees C. The detective wavelength was 230 nm.

RESULTS

The two enantiomers could be separated on the two kinds of chiral stationary phases without derivatization and the resolution was above 2.0. The methods developed on the two kinds of chiral stationary phases are accurate, sensitive and specific.

CONCLUSION

Both the proteinate- and amylose-based chiral stationary phases can be used to separate the enantiomers of cetirizine.

摘要

目的

研究盐酸西替利嗪在基于蛋白盐和直链淀粉的手性固定相上的色谱行为，以优化其对映体分离的色谱条件。

方法

使用基于直链淀粉、α1-酸性糖蛋白和卵类粘蛋白的手性固定相时，流动相分别为正己烷-异丙醇-乙醇-三氟乙酸（430:45:25:1）、乙腈-10 mmol·L⁻¹磷酸盐缓冲溶液（用氢氧化钠调至pH 7.0）（4:96）和乙腈-20 mmol·L⁻¹KH₂PO₄溶液（用三乙胺调至pH 7.0）（12.7:87.3）。蛋白盐柱温度为25℃，检测波长为230 nm。