整体柱支撑亲和色谱的建模
Modeling of monolith-supported affinity chromatography.
作者信息
Montes Sanchez Francisco J, Martin del Valle Eva, Galan Serrano Miguel A, Cerro Ramon L
机构信息
Department of Chemical and Materials Engineering, University of Alabama in Huntsville, Huntsville, Alabama 35899, USA.
出版信息
Biotechnol Prog. 2004 May-Jun;20(3):811-7. doi: 10.1021/bp034343n.
Ceramic monoliths have been used successfully as active support for affinity chromatography (1). A mathematical model was developed to simulate the adsorption-elution experimental behavior of asparaginase in an agarose-coated monolith support. The computer-based model allows precise determination of experimental parameters. Because of the simple geometry of ceramic monoliths used as support, the mathematical model can be used to design adsorption/elution cycles for the large throughput separation of biomolecules.
陶瓷整体柱已成功用作亲和色谱的活性载体(1)。建立了一个数学模型来模拟天冬酰胺酶在琼脂糖包被的整体柱载体中的吸附-洗脱实验行为。基于计算机的模型可以精确确定实验参数。由于用作载体的陶瓷整体柱几何形状简单，该数学模型可用于设计生物分子大通量分离的吸附/洗脱循环。
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