水凝胶对牛血清白蛋白吸附与回收的优化

Optimization of bovine serum albumin sorption and recovery by hydrogels.

作者信息

Gündüz Ufuk, Tolga Asli

机构信息

Kimya Mühendisliği Bölümü, Mühendislik ve Mimarlik Fakültesi, Gazi Universitesi, 06570 Ankara, Turkey.

出版信息

J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Jul 25;807(1):13-6. doi: 10.1016/j.jchromb.2004.01.060.

DOI:10.1016/j.jchromb.2004.01.060

PMID:15177154

Abstract

Aqueous two-phase systems are composed of aqueous solutions of either two water-soluble polymers, usually polyethylene glycol (PEG) and dextran (Dx), or a polymer and a salt, usually PEG and phosphate or sulfate. Partitioning of proteins in such systems provides a powerful method for separating and purifying mixtures of biomolecules by extraction. If one of the phase forming polymers is a crosslinked gel, then the solution-controlled gel sorption may be considered as a modification of aqueous two-phase extraction. Since PEG/dextran systems are widely used in aqueous two-phase extraction and dextran gels (Sephadex) are common chromatographic media, we choose a PEG/dextran gel system as a model system in this study. The partitioning behavior of pure bovine serum albumin (BSA) in PEG/dextran gel systems is investigated to see the effects of variations in PEG and NaCl concentrations on the partition coefficient K. By making use of the Box-Wilson experimental design, K is shown to be maximized at 9.8 (%, w/w) PEG and 0.2 M NaCl concentrations, respectively, as 182.

摘要

双水相系统由两种水溶性聚合物（通常是聚乙二醇（PEG）和葡聚糖（Dx））或一种聚合物与一种盐（通常是PEG与磷酸盐或硫酸盐）的水溶液组成。蛋白质在这类系统中的分配为通过萃取分离和纯化生物分子混合物提供了一种强大的方法。如果形成相的聚合物之一是交联凝胶，那么溶液控制的凝胶吸附可被视为双水相萃取的一种改进。由于PEG/葡聚糖系统在双水相萃取中广泛使用，且葡聚糖凝胶（葡聚糖凝胶）是常见的色谱介质，因此在本研究中我们选择PEG/葡聚糖凝胶系统作为模型系统。研究了纯牛血清白蛋白（BSA）在PEG/葡聚糖凝胶系统中的分配行为，以考察PEG和NaCl浓度变化对分配系数K的影响。通过使用Box-Wilson实验设计，结果表明在PEG浓度为9.8（%，w/w）和NaCl浓度为0.2 M时，K分别达到最大值182。