Singh Vijendra P, Srivastava N C, Kumar Manoj, Sunder M Jai, Varshney J P
National Referral Laboratory on Mycoplasma, Division of Bacteriology and Mycology, Indian Veterinary Research Institute, Izatnagar, UP, India.
Comp Immunol Microbiol Infect Dis. 2004 Jul;27(4):273-84. doi: 10.1016/j.cimid.2003.12.002.
Isolation and characterisation of an Indian strain of Mycoplasma mycoides subsp. mycoides LC from a case of caprine arthritis is reported in the study. The isolate was identified based on biochemical, digitonin sensitivity and growth inhibition tests. The virulence of the organism was studied by pathogenicity test in mice and goat. The antigenic and genomic profile of the isolate was compared with that of the standard strain (Y-Goat). Using different sets of primers, polymerase chain reaction was employed for rapid detection of the strain.
该研究报告了从一例山羊关节炎病例中分离并鉴定出一株印度丝状支原体丝状亚种LC。通过生化、皂角苷敏感性和生长抑制试验对分离株进行鉴定。通过在小鼠和山羊身上进行致病性试验研究该生物体的毒力。将分离株的抗原和基因组图谱与标准菌株(Y-山羊)进行比较。使用不同的引物组,采用聚合酶链反应对该菌株进行快速检测。