[中国杭州临床患者及环境分离副溶血性弧菌菌株毒力基因特征]
[Characteristics of virulence gene in Vibrio parahaemolyticus strains isolated from clinical patients and environment in Hangzhou, China].
作者信息
Zhang Wei, Meng Dong-mei, Pan Jing-cao, Zhu Feng-ying, Chen Kun
机构信息
Department of Epidemiology, School of Medicine, Zhejiang University, Hangzhou 310006, China.
出版信息
Zhonghua Yu Fang Yi Xue Za Zhi. 2004 May;38(3):200-3.
OBJECTIVES
To investigate the characteristics of virulence gene in Vibrio parahaemolyticus strains isolated from clinical patients and environment in Hangzhou, China.
METHODS
Thermostable direct hemolysin gene (tdh) and thermostable direct hemolysin-related hemolysin gene (trh) were determined in a total of 174 strains of V. parahaemolyticus isolated from patients and environment (seafood) in Hangzhou area by PCR.
RESULTS
The tdh was found in 92 out of 94 V. parahaemolyticus strains from food poisoning patients and in 33 out of 34 strains from sporadic diarrhea patients, and trh was not detected in all above clinical strains. Meanwhile the tdh was negative in all V. parahaemolyticus strains from environment, and the trh was also negative except one strain with urease activity. All strains with trh negative had no the activity of urease.
CONCLUSIONS
The V. parahaemolyticus strains from food poisoning patients and sporadic diarrhea patients are tdh positive and trh negative. The V. parahaemolyticus strains with tdh negative and almost trh positive in environment might be a potential pathogen in Hangzhou.
目的
调查从中国杭州临床患者和环境中分离的副溶血性弧菌菌株的毒力基因特征。
方法
采用聚合酶链反应（PCR）对从杭州地区患者和环境（海鲜）中分离出的174株副溶血性弧菌进行耐热直接溶血素基因（tdh）和耐热直接溶血素相关溶血素基因（trh）检测。
结果
在94株食物中毒患者分离的副溶血性弧菌中有92株检测到tdh，34株散发性腹泻患者分离的菌株中有33株检测到tdh，上述所有临床菌株均未检测到trh。同时，环境来源的所有副溶血性弧菌菌株tdh均为阴性，除1株具有脲酶活性的菌株外，trh也均为阴性。所有trh阴性菌株均无脲酶活性。
结论
食物中毒患者和散发性腹泻患者分离的副溶血性弧菌菌株tdh阳性、trh阴性。环境中tdh阴性且几乎trh阳性的副溶血性弧菌菌株可能是杭州地区的潜在病原体。
Zotero 插件