[NK细胞促进小鼠MHC半相合骨髓移植后供体骨髓植入及造血重建的实验研究]

[Experimental study on NK cells promoting donor marrow engraftment and hematopoietic reconstitution after MHC haploidentical BMT in mice].

作者信息

Wang Chun-yan, Guo Kun-yuan, Wu Bing-yi, Wu Lan-xiao, Jiang Zhen-yu, Pan Xing-hua

机构信息

The Department of Hematology, the Zhujiang Hospital of the First Military University, Guangzhou 510282, China.

出版信息

Zhonghua Xue Ye Xue Za Zhi. 2004 May;25(5):290-2.

PMID:15182539

Abstract

OBJECTIVE

To explore the effect of donor-derived NK cells added to pretreatment conditioning regimen on hematopoietic reconstitution after MHC haplotype-mismatched BMT in mice.

METHODS

Murine model of MHC haplotype-mismatched BMT was established by using BALB/c(H-2d) x C57BL/6(H-2b) (CB6F(1)(H-2d/b)) mouse as recipient, and C57BL/6(H-2b) mouse as donor. Fifty recipient mice were divided into 5 groups. The mice in the first three groups were each infused 1 x 10(6), 5 x 10(5), 2 x 10(5)/mouse donor-derived NK cells, respectively before TBI ((60)Co, 9.0 Gy) and then conditioned with TBI, followed by infusion of C57BL/6(H-2b) mice bone marrow cells four hours later. The mice in the fourth group received TBI only, and in the fifth group, TBI and BMT at the some doses as the first three groups. Hematopoietic reconstitution, survival time, body weight, histopathology of the recipients were followed up.

RESULTS

(1) Survival time was (5.15 +/- 0.66) days for the fourth group, and > 30 days for the other 4 groups. (2) Leukocyte and platelet counts at day 10 after BMT were (0.99 +/- 0.22) x 10(9)/L and (61.0 +/- 7.27) x 10(9)/L respectively for the fifth group and (2.01 +/- 0.21) x 10(9)/L, (101.50 +/- 16.34) x 10(9)/L; (1.98 +/- 0.29) x 10(9)/L, (99.50 +/- 16.41) x 10(9)/L and (1.97 +/- 0.21) x 10(9)/L, (98.0 +/- 16.19) x 10(9)/L for the first three groups, respectively. Histopathology displayed no GVHD in all the groups.

CONCLUSION

Donor-derived NK cells could promote hematopoietic reconstitution after MHC haplotype-mismatched BMT in mice.

摘要

目的

探讨在预处理方案中加入供体来源的自然杀伤（NK）细胞对小鼠主要组织相容性复合体（MHC）单倍型不相合骨髓移植（BMT）后造血重建的影响。

方法

以BALB/c（H-2d）×C57BL/6（H-2b）（CB6F1（H-2d/b））小鼠为受体，C57BL/6（H-2b）小鼠为供体，建立MHC单倍型不相合BMT小鼠模型。50只受体小鼠分为5组。前三组小鼠在全身照射（TBI，（60）Co，9.0 Gy）前分别每只输注1×10⁶、5×10⁵、2×10⁵个供体来源的NK细胞，然后进行TBI预处理，4小时后输注C57BL/6（H-2b）小鼠骨髓细胞。第四组小鼠仅接受TBI，第五组小鼠接受与前三组相同剂量的TBI和BMT。对受体小鼠的造血重建、生存时间、体重、组织病理学进行随访。

结果

（1）第四组小鼠的生存时间为（5.15±0.66）天，其他4组小鼠的生存时间>30天。（2）BMT后第10天，第五组小鼠的白细胞计数和血小板计数分别为（0.99±0.22）×10⁹/L和（61.0±7.27）×10⁹/L，前三组小鼠的白细胞计数和血小板计数分别为（2.01±0.21）×10⁹/L、（101.50±16.34）×10⁹/L；（1.98±0.29）×10⁹/L、（99.50±16.41）×10⁹/L和（1.97±0.21）×10⁹/L、（98.0±16.19）×10⁹/L。组织病理学检查显示所有组均无移植物抗宿主病（GVHD）。