[采用序列特异性引物聚合酶链反应进行HLA-DR DNA分型]
[Typing for HLA-DR DNA by polymerase chain reaction with sequence-specific primers].
作者信息
Wu Guo-jun, Wang He, Yu Lei, Yuan Jian-lin, Zhang Bo, Guo Wei, Wang Dong, Li Xin
机构信息
Department of Urology Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
出版信息
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2003 Nov;19(6):560-2.
AIM
To type the HLA-DR DNA for renal transplantation by PCR with sequence specific primers (PCR-SSP).
METHODS
According to nucleotide sequences of HLA-DR, 16 pairs of specific primers and a pair of positive control primers were designed and synthesized for PCR-SSP. Then HLA-DR sites of 52 donors and recipients for renal transplantation were typed by the PCR-SSP.
RESULTS
All the samples were successfully typed by PCR-SSP with the synthesized primers. The results were available within 3 hours after sampling and the accuracy and reproducibility were 100%.
CONCLUSION
Genotyping for HLA-DR sites by PCR-SSP with primers reported herein was a simple and accurate technique suitable for clinical application.
目的
采用序列特异性引物聚合酶链反应（PCR-SSP）对肾移植患者进行HLA-DR基因分型。
方法
根据HLA-DR的核苷酸序列，设计并合成16对特异性引物及1对阳性对照引物用于PCR-SSP。然后采用PCR-SSP对52例肾移植供受者的HLA-DR位点进行分型。
结果
用合成的引物通过PCR-SSP成功对所有样本进行了分型。取样后3小时内即可获得结果，准确性和重复性均为100%。
结论
采用本文报道的引物通过PCR-SSP对HLA-DR位点进行基因分型是一种简单、准确的技术，适用于临床应用。
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