Engineered FGF-2 expression induces glandular epithelial hyperplasia in the murine prostatic dorsal lobe.

作者信息

Konno-Takahashi Naoko, Takeuchi Takumi, Nishimatsu Hiroaki, Kamijo Toshiyuki, Tomita Kyoichi, Schalken Jack A, Teshima Shinichi, Kitamura Tadaichi

机构信息

Department of Urology and Andrology, Faculty of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan.

出版信息

Eur Urol. 2004 Jul;46(1):126-32. doi: 10.1016/j.eururo.2004.02.004.

DOI:10.1016/j.eururo.2004.02.004

PMID:15183558

Abstract

OBJECTIVE

It is known that androgens and stromal-epithelial interactions are required for the formation and growth of the prostate. FGF-2 is overexpressed in prostatic stromal cells in benign prostatic hypertrophy (BPH)/prostate cancer. This supports the paracrine/autocrine growth of prostatic epithelial/stromal cells in the pathogenesis of BPH and invasive prostate cancer.

METHODS

We established transgenic mice expressing FGF-2 under the control of a short rat probasin promotor. FGF-2 transgenic founder mice expressing FGF-2 in the prostate were infertile. Thus, male founder mice were sacrificed for histological analysis.

RESULTS

FGF-2 was expressed in epithelial cells in glands of the dorsal, lateral, and ventral prostatic lobes of two FGF-2 transgenic founder mice, but not in the anterior lobe of transgenic mice or in any lobe of non-transgenic control littermates. Acinar epithelial glands in dorsal prostatic lobes of FGF-2 transgenic mice expressing FGF-2 were more dense and showed simple papillary hyperplasia of epithelial cells compared with those of control littermate mice. Glandular and luminal enlargement without epithelial growth was observed in the ventral lobe of FGF-2 transgenic mice compared with the controls.

CONCLUSION

In conclusion, FGF-2 transgenic mice under the control of rat probasin promoter showed simple epithelial hyperplasia in glands of the prostatic dorsal lobe and glandular enlargement without epithelial growth in the prostatic ventral lobe.

摘要