Love Sherie L, Manlandro Cara Marie A, Testa Christopher J, Thomas Anne E, Tryggestad Kari-Elise, Rosenwald Anne G
Department of Biology, Georgetown University, Washington, DC 20057, USA.
Biochem Biophys Res Commun. 2004 Jul 2;319(3):840-6. doi: 10.1016/j.bbrc.2004.05.063.
The yeast ARL1 gene, encoding a guanine-nucleotide binding protein of the Arf-like family, exhibits a synthetic genetic interaction with CCZ1. An arl1 Delta ccz1 Delta double mutant was viable but grew slowly, was more sensitive to caffeine, Ca(2+), Zn(2+), and hygromycin B than either single mutant, and had a more severe vacuolar protein sorting phenotype. Overexpression of ARL1 did not suppress ccz1 Delta mutant phenotypes, nor did overexpression of CCZ1 suppress arl1 Delta mutant phenotypes. We conclude that ARL1 and CCZ1 independently contribute to both ion homeostasis and protein sorting.
酵母ARL1基因编码一种Arf样家族的鸟嘌呤核苷酸结合蛋白，它与CCZ1表现出合成遗传相互作用。arl1Δccz1Δ双突变体是有活力的，但生长缓慢，对咖啡因、Ca(2+)、Zn(2+)和潮霉素B比任一单突变体更敏感，并且具有更严重的液泡蛋白分选表型。ARL1的过表达不能抑制ccz1Δ突变体表型，CCZ1的过表达也不能抑制arl1Δ突变体表型。我们得出结论，ARL1和CCZ1独立地对离子稳态和蛋白分选起作用。
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