[使用多位点测序对流感嗜血杆菌菌株进行鉴定]

[Characterization of Haemophilus influenzae strains using multilocus sequencing].

作者信息

Krízová P, Kalmusová J, Lebedová V, Felsberg J, Haugvicová R

机构信息

Centrum epidemiologie a mikrobiologie, Státní zdravotní ústav, Praha.

出版信息

Epidemiol Mikrobiol Imunol. 2004;53(2):74-7.

PMID:15185633

Abstract

First results of multilocus sequence typing (MLST) of Haemophilus influenzae strains are presented. MLST of 28 H. influenzae strains isolated from patients with invasive diseases in the Czech Republic is indicative of clonal homogeneity of these strains: 22 out of 26 H. influenzae b strains tested were of the same sequence type, ST-6. Four strains were of two sequence types newly described in this study: ST-83 (3 strains) and ST-84 (1 strain). Two nontypeable H. influenzae strains were assigned to sequence types other than ST-6: ST-3 and ST-85 newly described in this study. First MLST results show ST-6 to be typical of H. influenzae b isolated from patients with invasive diseases in the Czech Republic. The sequence types newly described in this study, i.e. ST-83, ST-84 and ST-85, were submitted to the worldwide H. influenzae MLST database (http://haemophilus.mlst.net).

摘要

本文展示了流感嗜血杆菌菌株多位点序列分型（MLST）的初步结果。对从捷克共和国侵袭性疾病患者中分离出的28株流感嗜血杆菌进行的MLST表明，这些菌株具有克隆同质性：在检测的26株b型流感嗜血杆菌中，有22株属于同一序列类型，即ST-6。有4株属于本研究新描述的两种序列类型：ST-83（3株）和ST-84（1株）。两株不可分型的流感嗜血杆菌菌株被归为ST-6以外的序列类型：本研究新描述的ST-3和ST-85。MLST的初步结果显示，ST-6是从捷克共和国侵袭性疾病患者中分离出的b型流感嗜血杆菌的典型序列类型。本研究新描述的序列类型，即ST-83、ST-84和ST-85，已提交至全球流感嗜血杆菌MLST数据库（http://haemophilus.mlst.net）。