[俄罗斯和哈萨克斯坦硬蜱中埃立克体和无形体检测的新证据]

[New evidence for the detection of ehrlichia and anaplasma in ixodes ticks in Russia and Kazakhstan].

作者信息

Shpynov S N, Rudakov N V, Iastrebov V K, Leonova G N, Khazova T G, Egorova N V, Borisova O N, Preĭder V P, Bezrukov G V, Fedorov E G, Fedianin A P, Sherstneva M B, Turyshev A G, Gavrilov A P, Tankibaev M A, Fournier P E, Raoult D

出版信息

Med Parazitol (Mosk). 2004 Apr-Jun(2):10-4.

PMID:15193042

Abstract

Polymerase chain reaction and sequence analysis were used to screen Ehrlichia and Anaplasma DNA in 900 specimens of Ixodes ticks of four genera (Dermacetor, Haemaphysalis, Ixodes, and Hyalomma) collected on 14 administrative territories of Russia and Kazakhstan. Anaplasma phagocytophilia DNA was detected and identified in Ixodes persulcatus ticks gathered in the Altai and Primorye Territories. Ehrlichia muris DNA was genotyped in the I. persulcatus ticks collected in the Tyumen, Omsk, and Novosibirsk Regions and the Altai Territory. Examining the Haemaphysalis concinna collected in the Primorye Territory revealed A. bovis DNA. The I. persulcatus ticks gathered in the Omsk Region were found to have "Ehrlichia-like "Schotti variant" DNA, Studying the ticks of the genera Dermatocentor and Hyalomma gathered in Russia and Kazakhstan failed to detect the representatives of the genera Anaplasma and Ehrlichia. The detection of A. phagocytophilia DNA in the I. persulcatus ticks in the Altai and Primorye territories and the serological verification of cases of human granulocytic anaplasmosis among patients with tick-borne infections in these territories lead to the conclusion that there are natural loci of this disease in West Siberia and the Far East of Russia.

摘要

采用聚合酶链反应和序列分析方法，对在俄罗斯和哈萨克斯坦14个行政区采集的900份四个属（革蜱属、血蜱属、硬蜱属和璃眼蜱属）硬蜱标本中的埃立克体属和无形体属DNA进行筛查。在阿尔泰地区和滨海边疆区采集的全沟硬蜱中检测并鉴定出嗜吞噬细胞无形体DNA。在秋明州、鄂木斯克州、新西伯利亚州和阿尔泰边疆区采集的全沟硬蜱中对鼠埃立克体DNA进行了基因分型。对滨海边疆区采集的日本血蜱进行检测，发现了牛无形体DNA。在鄂木斯克州采集的全沟硬蜱中发现有“类埃立克体‘肖蒂变种’”DNA。对在俄罗斯和哈萨克斯坦采集的革蜱属和璃眼蜱属蜱虫进行研究，未检测到无形体属和埃立克体属的代表。在阿尔泰地区和滨海边疆区的全沟硬蜱中检测到嗜吞噬细胞无形体DNA，并且对这些地区蜱传感染患者中的人类粒细胞无形体病病例进行了血清学验证，从而得出结论，在俄罗斯西西伯利亚和远东地区存在该病的自然疫源地。