正常人和葡萄糖-6-磷酸脱氢酶（G6PD）缺乏者的G6PD动力学研究及某些药物的影响。

Investigation of glucose 6-phosphate dehydrogenase (G6PD) kinetics for normal and G6PD-deficient persons and the effects of some drugs.

作者信息

Ozmen Ismail, Ciftçi Mehmet, Küfrevioğlu O Irfan, Curük M Akif

机构信息

Biotechnology Application and Research Center, Atatürk University, 25240 Erzurum, Turkey.

出版信息

J Enzyme Inhib Med Chem. 2004 Feb;19(1):45-50. doi: 10.1080/1475636032000141917.

DOI:10.1080/1475636032000141917

PMID:15202492

Abstract

In the present study, blood samples from 1183 children aged 0.5-6 years were taken. Three children were found with G6PD deficiency by examining the enzyme activity and hemoglobin ratio. Some kinetic properties of glucose 6-phosphate dehydrogenase enzyme (G6PD) were studied after the purification of the enzyme with ammonium fractionation, dialysis and 2',5' ADP-Sepharose 4B affinity chromatography from a healthy person and from three G6PD-deficient people. The purity of the enzymes was confirmed by SDS-PAGE electrophoresis. The effects of some drugs which are known inhibitors of G6PD activity were studied. Some of the drugs stimulated the activity of the enzyme in two of the three cases with G6PD deficiency. KM values, Vmax values for G6P and NADP+, optimum pH and optimum temperature for the enzyme from the healthy person and the three G6PD-defficient people are reported.

摘要

在本研究中，采集了1183名0.5至6岁儿童的血样。通过检测酶活性和血红蛋白比例，发现3名儿童患有葡萄糖-6-磷酸脱氢酶（G6PD）缺乏症。在用硫酸铵分级分离、透析以及2',5'-二磷酸腺苷-琼脂糖4B亲和层析法从一名健康人和三名G6PD缺乏者体内纯化该酶后，研究了葡萄糖-6-磷酸脱氢酶（G6PD）的一些动力学特性。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）确认了酶的纯度。研究了一些已知的G6PD活性抑制剂药物的作用。在三名G6PD缺乏者中的两名中，某些药物刺激了该酶的活性。报告了来自健康人和三名G6PD缺乏者的该酶对于葡萄糖-6-磷酸（G6P）和烟酰胺腺嘌呤二核苷酸磷酸（NADP+）的米氏常数（KM值）、最大反应速度（Vmax值）、最适pH值和最适温度。