Amini Hossein, Shahmir Badri, Ahmadiani Abolhassan
Department of Pharmacology, Neuroscience Research Center, Shaheed Beheshti University of Medical Sciences, P.O. Box 19835-355, Tehran, Iran.
J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Aug 5;807(2):271-5. doi: 10.1016/j.jchromb.2004.04.016.
A simple and sensitive high-performance liquid chromatographic (HPLC) method with spectrophotometric detection was developed for the determination of moclobemide in human plasma. Plasma samples were extracted under basic conditions with dichloromethane followed by back-extraction into diluted phosphoric acid. Isocratic separation was employed on an ODS column (250 mm x 4.6 mm, 5 microm) at room temperature. The mobile phase consisted of 5 mM NaH2PO4-acetonitrile-triethylamine (1000:350:10 (v/v/v), pH 3.4). Analyses were run at a flow-rate of 1.0 ml/min and ultraviolet (UV) detection was carried out at 240 nm. The method was specific and sensitive with a quantification limit of 15.6 ng/ml and a detection limit of 5 ng/ml at a signal-to-noise ratio of 3:1. The mean absolute recovery was about 98.2%, while the intra- and inter-day coefficient of variation and percent error values of the assay method were all at acceptable levels. Linearity was assessed in the range of 15.6-2000 ng/ml in plasma with a correlation coefficient of greater than 0.999. This method has been used to analyze several hundred human plasma samples for bioavailibility studies.
建立了一种采用分光光度检测的简单、灵敏的高效液相色谱（HPLC）法，用于测定人血浆中的吗氯贝胺。血浆样品在碱性条件下用二氯甲烷萃取，然后反萃取到稀释的磷酸中。在室温下，采用ODS柱（250 mm×4.6 mm，5μm）进行等度分离。流动相由5 mM磷酸二氢钠 - 乙腈 - 三乙胺（1000:350:10（v/v/v），pH 3.4）组成。分析以1.0 ml/min的流速进行，在240 nm处进行紫外（UV）检测。该方法特异性强、灵敏度高，定量限为15.6 ng/ml，信噪比为3:1时检测限为5 ng/ml。平均绝对回收率约为98.2%，该测定方法的日内和日间变异系数以及误差百分比值均处于可接受水平。在血浆中15.6 - 2000 ng/ml范围内评估线性，相关系数大于0.999。该方法已用于分析数百份人血浆样品以进行生物利用度研究。
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