四环素和四环素阻遏物变体对大肠杆菌中两个基因的独立调控
Independent regulation of two genes in Escherichia coli by tetracyclines and Tet repressor variants.
作者信息
Kamionka Annette, Sehnal Miriam, Scholz Oliver, Hillen Wolfgang
机构信息
Lehrstuhl für Mikrobiologie, Biochemie und Genetik, Friedrich-Alexander-Universität Erlangen-Nürnberg, 91058 Erlangen, Germany.
出版信息
J Bacteriol. 2004 Jul;186(13):4399-401. doi: 10.1128/JB.186.13.4399-4401.2004.
Abstract
We report a regulation system in Escherichia coli for independent regulation of two distinct reporter genes by application of Tet repressors with different specificities. One Tet repressor variant comprises wild-type tet operator (tetO) recognition and exclusive induction with the novel inducer 4-dedimethylamino-anhydrotetracycline. The other Tet repressor variant shows tetO-4C recognition and induction with tetracycline. We demonstrate that both variants are independently active in vivo and allow selective regulation of two genes in the same cell without any cross talk.
摘要
我们报道了一种大肠杆菌中的调控系统，通过应用具有不同特异性的四环素阻遏物来独立调控两个不同的报告基因。一种四环素阻遏物变体包含野生型四环素操纵子（tetO）识别序列，并仅由新型诱导剂4-去二甲基氨基-脱水四环素诱导。另一种四环素阻遏物变体表现出对tetO-4C的识别，并由四环素诱导。我们证明这两种变体在体内均具有独立活性，并且能够在同一细胞中对两个基因进行选择性调控，而不存在任何串扰。
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