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在储存三天、七天和十天后,将Safe2O家禽清洗液应用于肉鸡翅膀对其保质期、单核细胞增生李斯特菌、假单胞菌、葡萄球菌属和嗜冷菌水平的影响。

Effects of applying Safe2O poultry wash to broiler wings on shelf life, Listeria monocytogenes, Pseudomonads, Staphylococcus species, and psychrotrophic bacteria levels after three, seven, and ten days of storage.

作者信息

Dickens J A, Ingram K D, Hinton A

机构信息

USDA, ARS, SAA, Russell Research Center, Athens, Georgia 30604, USA.

出版信息

Poult Sci. 2004 Jun;83(6):1047-50. doi: 10.1093/ps/83.6.1047.

Abstract

Bacterial contamination of raw processed poultry continues to be of concern to consumers as well as regulatory and health officials. For many years wings were considered a low-value product; therefore, shelf life of wings was not a major concern. Due to changes in consumer attitudes and increases in the fast-food market, wings are now a valuable commodity. Because wings have a shorter shelf life than most other raw poultry products, acceptable intervention to decrease the population of associated spoilage organisms and human enteropathogens are needed. Safe2O Poultry Wash was evaluated as a postchill treatment to reduce microbial contamination and increase shelf life. Ninety-six carcasses were obtained from a local processor prior to final wash. On arrival at the research facility all carcasses were inoculated with 1 mL of a culture with 10(3) cfu/mL Listeria monocytogenes. After a 30-min attachment time, carcasses were subjected to a 4-s in-out final wash, hung for 3 min, and chilled in ice-water for 45 min. After the chilling, wings were removed by hand with a knife, pooled together, and subjected to a hand spray (4 mL/wing) with deionized water or Safe2O Poultry Wash. Two wings were then placed in each of 96 ziplock type storage bags, and wings were held at 5 +/- 1 degrees C for 3, 7, 10, and 14 d. On the day of sample, weep was decanted, and 100 mL of Butterfield's phosphate buffer was added to each bag. Three sets of wings were shaken by hand for 1 min, and total aerobes, Pseudomonads, Staphylococcus sp., psychrotrophic bacteria, and L. monocytogenes in the rinsates were enumerated. By using 7 log10 recovery of total aerobes from rinsates as a spoilage baseline, all wings were spoiled by d 10, but the wings treated with water were approaching spoilage counts on d 7, (log10 6.8), whereas only log10 5.5 bacteria were recovered from the wings sprayed with Safe2O Poultry Wash. Fewer Pseudomonads, Staphylcoccus sp., L. monocytogenes, and psychrotrophic bacteria were recovered from wings treated with Safe2O Poultry Wash and stored for 10 d. Log10 counts for the organisms were Pseudomonas sp., 8.2 and 6.9; Staphylcoccus sp., 5.5 and 4.9; L. monocytogenes, 5.2 and 4.6; and psychrotrophs, 8.2 and 6.9 for the water and Safe2O Poultry Wash treatments, respectively. Use of the Safe2O Poultry Wash as a postchill treatment on wings could increase the shelf life of wings by up to 3 d.

摘要

生加工家禽的细菌污染一直是消费者、监管及卫生官员所关注的问题。多年来,鸡翅被视为低价值产品;因此,鸡翅的保质期并非主要关注点。由于消费者态度的转变以及快餐市场的增长,如今鸡翅成为了一种有价值的商品。因为鸡翅的保质期比大多数其他生禽产品短,所以需要采取可接受的干预措施来减少相关腐败微生物和人类肠道病原体的数量。对Safe2O家禽清洗液进行了评估,作为一种冷却后处理方法,以减少微生物污染并延长保质期。在最终清洗之前,从当地加工厂获取了96个禽体。到达研究设施后,所有禽体均接种1 mL含10³ cfu/mL单核细胞增生李斯特菌的培养物。经过30分钟的附着时间后,禽体进行4秒的进出式最终清洗,悬挂3分钟,然后在冰水中冷却45分钟。冷却后,用刀手工切下鸡翅,汇集在一起,并用去离子水或Safe2O家禽清洗液进行手工喷雾(4 mL/鸡翅)。然后将两只鸡翅放入96个拉链式储存袋中的每一个中,鸡翅在5±1℃下保存3、7、10和14天。在取样当天,倒出渗出液,向每个袋子中加入100 mL巴特菲尔德磷酸盐缓冲液。三组鸡翅手工摇晃1分钟,对冲洗液中的需氧菌总数﹑假单胞菌﹑葡萄球菌属﹑嗜冷菌和单核细胞增生李斯特菌进行计数。以冲洗液中需氧菌总数7 log₁₀的回收率作为腐败基线,所有鸡翅在第10天均已变质,但用水处理的鸡翅在第7天(log₁₀ 6.8)已接近变质菌数,而用Safe2O家禽清洗液喷雾处理的鸡翅仅回收了log₁₀ 5.5个细菌。从用Safe2O家禽清洗液处理并储存10天的鸡翅中回收的假单胞菌、葡萄球菌属、单核细胞增生李斯特菌和嗜冷菌较少。这些微生物的log₁₀菌数分别为:假单胞菌属,水和Safe2O家禽清洗液处理组分别为8.2和6.9;葡萄球菌属,5.5和4.9;单核细胞增生李斯特菌,5.2和4.6;嗜冷菌,8.2和6.9。将Safe2O家禽清洗液用作鸡翅冷却后处理可使鸡翅的保质期延长多达3天。

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