[从C7C噬菌体展示肽库中筛选BLyS拮抗剂肽]
[Screening of antagonist peptide of BLyS from C7C phage display peptide library].
作者信息
Ji Qing, He Feng-tian, Li Rong-fen, Gao Hui-guang, Zheng Ying-ru, Zhong Xiao-lin, Zheng Han-qi
机构信息
Department of Biochemistry & Molecular Biology, Third Military Medical University, Chongqing 400038, China.
出版信息
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2004 Jul;20(4):398-401.
AIM
To screen an antagonist peptide of BLyS from C7C phage display peptide library.
METHODS
C7C phage display peptide library was screened with BLyS. Indirect ELISA, competitive ELISA and MTT colorimetry were used to identify positive phage clones.
RESULTS
After 3 rounds of screening, the gradual increase of the ratio of output to input and specific enrichment had been achieved. Two phage clones that could inhibit the BLyS activity were identified.
CONCLUSION
Two phage display antagonist peptides of BLyS were obtained.
目的
从C7C噬菌体展示肽库中筛选B淋巴细胞刺激因子（BLyS）的拮抗肽。
方法
用BLyS筛选C7C噬菌体展示肽库。采用间接酶联免疫吸附测定（ELISA）、竞争性ELISA和MTT比色法鉴定阳性噬菌体克隆。
结果
经过3轮筛选，实现了产出与投入比的逐步增加和特异性富集。鉴定出两个可抑制BLyS活性的噬菌体克隆。
结论
获得了两个BLyS噬菌体展示拮抗肽。
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