用于光固定微阵列的光反应性聚乙烯醇。

Photo-reactive polyvinylalcohol for photo-immobilized microarray.

作者信息

Ito Yoshihiro, Nogawa Masayuki, Takeda Mineko, Shibuya Tohru

机构信息

Kanagawa Academy of Science and Technology, Regenerative Medical Bioreactor Project, KSP East 309, 3-2-1 Sakado, Takatsu-ku, Kawasaki, Kanagawa 213-0012, Japan.

出版信息

Biomaterials. 2005 Jan;26(2):211-6. doi: 10.1016/j.biomaterials.2004.02.019.

DOI:10.1016/j.biomaterials.2004.02.019

PMID:15207468

Abstract

A new photo-reactive polymer, polyvinylalcohol modified with phenylazido groups, was synthesized as a microarray matrix. The polymer is soluble in water and spin-coated onto glass plate. Aqueous solutions of proteins were micro-spotted onto the coated glass and were fixed by ultraviolet light irradiation. Subsequently, cell adhesion on the photo-immobilized protein microarray was investigated. Non-specific adhesion of cells onto non-protein-spotted regions was reduced in comparison with the previously prepared microarray chip (Biomaterials 24 (2003) 3021). The adhesion behavior of cells depended on the kind of immobilized proteins and the type of cells. The microarray will be useful for cell diagnosis and for the selection of biomaterials to regulate cell behavior.

摘要

一种新型的光反应性聚合物，即经苯基叠氮基修饰的聚乙烯醇，被合成为一种微阵列基质。该聚合物可溶于水，并通过旋涂法涂覆在玻璃板上。将蛋白质水溶液微点样到涂覆好的玻璃上，然后通过紫外线照射进行固定。随后，研究了细胞在光固定化蛋白质微阵列上的黏附情况。与之前制备的微阵列芯片相比，细胞在非蛋白质点样区域的非特异性黏附减少了（《生物材料》24 (2003) 3021）。细胞的黏附行为取决于固定化蛋白质的种类和细胞类型。这种微阵列将有助于细胞诊断以及用于选择调节细胞行为的生物材料。