Kotsofliak O I, Reva O M, Tashyrev O B
Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, Kyiv.
Mikrobiol Z. 2004 Mar-Apr;66(2):3-10.
Fifty (50) strains of Pseudomonas genus have been isolated from the samples of soil, moss and bottom sediments taken on the Galindes, Piterman and Deception Islands (Ukrainian Antarctic station Akademik Vernadsky). Characteristic of the above strains by 84 phenotype features and identification by means of the computer program permitted them to be referred to the species Pseudomonas fluorescens (biovar V.), P. putida (biovars A and B), P. veronii. The belonging of the strain 23 to the species P. veronii has been confirmed by the results of sequence of 16S rRNA. Being grown at low temperatures (4 degrees C) the strains of the above species are characterized by high antagonistic activity as to Gram-positive, Gram-negative bacteria, yeast and microscopic fungi. Cultivation of antagonists at 26 degrees C led to the sharp decrease or complete disappearance of the antagonistic effect.
从加林德斯岛、皮特曼岛和欺骗岛(乌克兰南极站“弗纳德斯基院士”)采集的土壤、苔藓和底部沉积物样本中分离出了50株假单胞菌属菌株。通过84种表型特征对上述菌株进行表征,并借助计算机程序进行鉴定,结果表明它们分别属于荧光假单胞菌(生物变种V)、恶臭假单胞菌(生物变种A和B)、维罗纳假单胞菌。16S rRNA序列分析结果证实了23号菌株属于维罗纳假单胞菌。上述菌种的菌株在低温(4摄氏度)下生长时,对革兰氏阳性菌、革兰氏阴性菌、酵母和显微真菌具有高拮抗活性。在26摄氏度下培养拮抗菌会导致拮抗作用急剧下降或完全消失。