Wang Lin, Luo Qifang
Institute of Environmental Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Wei Sheng Yan Jiu. 2004 Mar;33(2):137-9.
In order to screen the dominant bacteria for degrading endocrine disruptor di-n-butyl phthalate (DBP).
The activated sludge was acclimated by DBP as sole carbon source. DBP concentration was increased progressively. 5 strains were isolated from the sludge by the plane-table after 10 weeks, 1 strain of which grew and degraded DBP well.
By orthogonal test, it was indicated that the appropriate degradation conditions of DBP for this best strain were degradation time 32 h, DBP concentration 200 mg/L, rate of shaking incubator 100 r/min, pH 7.0, temperature 30 degrees C. The degraded rate of DBP is more than 95% under above conditions.
The bacterium was identified as Pseudomonas.
筛选降解内分泌干扰物邻苯二甲酸二丁酯（DBP）的优势菌。
以DBP作为唯一碳源对活性污泥进行驯化，逐步提高DBP浓度。10周后通过平板法从污泥中分离出5株菌，其中1株生长良好且能降解DBP。
通过正交试验表明，该最优菌株降解DBP的适宜条件为：降解时间32小时、DBP浓度200毫克/升、摇床转速100转/分钟、pH值7.0、温度30℃。在此条件下DBP的降解率大于95%。
该菌鉴定为假单胞菌属。
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