Fujiu K, Numata O
Institute of Biological Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan.
Cell Struct Funct. 1999 Oct;24(5):401-4. doi: 10.1247/csf.24.401.
The ciliated protozoa Tetrahymena contains two nuclei, a micronucleus and a macronucleus. In the vegetatively growing cell, the macronucleus divides amitotic while the micronucleus divides by mitosis. It has been indicated that microtubules are involved in macronuclear division and microtubules are observed to exist in the dividing macronucleus. To clarify the localization and the organization of microtubules in the amitotic dividing macronuclei, we used immunofluorescent staining technique. The microtubules were observed in the cytoplasm and macronucleus. The microtubules were organized and dynamically changed their distribution throughout the macronuclear division. We suggest a possibility that these microtubules are involved in 'amitotic' distribution of chromatin throughout the macronuclear division.
纤毛原生动物四膜虫含有两个细胞核，即微核和大核。在营养生长细胞中，大核进行无丝分裂，而微核进行有丝分裂。已有研究表明微管参与大核分裂，并且在正在分裂的大核中观察到微管的存在。为了阐明微管在无丝分裂的大核中的定位和组织方式，我们使用了免疫荧光染色技术。在细胞质和大核中观察到了微管。微管在整个大核分裂过程中进行组织并动态改变其分布。我们提出一种可能性，即这些微管在整个大核分裂过程中参与染色质的“无丝分裂”分布。
