用于快速测定对乙酰氨基酚及其水解产物的聚二甲基硅氧烷微芯片毛细管电泳-电化学检测法

Poly(dimethylsiloxane) microchip capillary electrophoresis with electrochemical detection for rapid measurement of acetaminophen and its hydrolysate.

作者信息

He F Y, Liu A L, Xia X H

机构信息

The State Key Laboratory of Coordination Chemistry, Institute of Analytical Science, Department of Chemistry, Nanjing University, 210093 Nanjing, China.

出版信息

Anal Bioanal Chem. 2004 Aug;379(7-8):1062-7. doi: 10.1007/s00216-004-2680-4. Epub 2004 Jun 18.

DOI:10.1007/s00216-004-2680-4

PMID:15221194

Abstract

Poly(dimethylsiloxane) microchip capillary electrophoresis with amperometric detection has been used for rapid separation and determination of acetaminophen and its hydrolysate, i.e. p-aminophenol. A Pt ultramicroelectrode with a diameter of 10 microm positioned at the outlet of the separation channel was used as a working electrode for amperometric detection. Factors influencing separation and detection were investigated and optimized. Results show that acetaminophen and p-aminophenol can be well separated within 35 s with RSD-1 (approximately 0.1 fmol) at S/ N=3. This method has been successfully applied to the detection of traces of p-aminophenol in paracetamol tablets.

摘要

聚二甲基硅氧烷微芯片毛细管电泳结合安培检测法已用于对乙酰氨基酚及其水解产物即对氨基酚的快速分离和测定。将直径为10微米的铂超微电极置于分离通道出口处，用作安培检测的工作电极。对影响分离和检测的因素进行了研究并优化。结果表明，对乙酰氨基酚和对氨基酚可在35秒内实现良好分离，在信噪比为3时，相对标准偏差为1（约0.1飞摩尔）。该方法已成功应用于扑热息痛片中痕量对氨基酚的检测。