部分睡眠剥夺大鼠胃黏膜中诱导型热休克蛋白70的过表达

Over-expression of inducible heat shock protein 70 in the gastric mucosa of partially sleep-deprived rats.

作者信息

Guo J S, Chau J F L, Shen X Z, Cho C H, Luk J M, Koo M W L

机构信息

Dept. of Pharmacology, Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong.

出版信息

Scand J Gastroenterol. 2004 Jun;39(6):510-5. doi: 10.1080/00365520410004523.

DOI:10.1080/00365520410004523

PMID:15223672

Abstract

BACKGROUND

Previous findings have demonstrated increased expression of inducible heat shock protein 70 (iHSP70) in the gastric mucosa of rats exposed to partial sleep deprived (PSD). The purpose of this study was to investigate the functional role of iHSP70 and its relationship with acid secretion in the stomachs of PSD animals.

METHODS

A slowly rotating drum was used to induce PSD in male Sprague-Dawley rats with or without omeprazole treatment. Gastric mucosal samples were harvested for iHSP70 mRNA and protein analysis with RT-PCR and Western blotting, respectively. Enzyme immunoassay was used to determine plasma gastrin level and gastric acidity was measured by titration. The modulating effect of PSD on 0.6 M hydrochloric acid (HCl)-induced gastric damage was also evaluated.

RESULTS

PSD increased plasma gastrin, gastric acidity and expression of iHSP70, while significantly reducing HCl-induced gastric damage. Omeprazole administration decreased gastric acidity and reversed iHSP70 over-expression in PSD rats.

CONCLUSIONS

PSD increases gastric acidity which enhances expression of mucosal iHSP70. Over-expression of iHSP70 may be a protective homeostatic response of the stomach to stress induced by PSD and acid secretion.

摘要

背景

先前的研究结果表明，部分睡眠剥夺（PSD）大鼠胃黏膜中诱导型热休克蛋白70（iHSP70）的表达增加。本研究旨在探讨iHSP70在PSD动物胃中的功能作用及其与胃酸分泌的关系。

方法

使用缓慢旋转的鼓对雄性Sprague-Dawley大鼠进行PSD诱导，部分大鼠同时接受奥美拉唑治疗。分别通过逆转录聚合酶链反应（RT-PCR）和蛋白质印迹法对胃黏膜样本进行iHSP70 mRNA和蛋白质分析。采用酶免疫测定法测定血浆胃泌素水平，通过滴定法测量胃酸度。还评估了PSD对0.6 M盐酸（HCl）诱导的胃损伤的调节作用。