蛋白质 - DNA 界面的适应性：对 engrailed 同源结构域进行重新设计以识别非天然核苷酸。

Adaptability at a protein-DNA interface: re-engineering the engrailed homeodomain to recognize an unnatural nucleotide.

作者信息

Simon Matthew D, Shokat Kevan M

机构信息

Department of Chemistry, University of California, Berkeley, CA 94720, USA.

出版信息

J Am Chem Soc. 2004 Jul 7;126(26):8078-9. doi: 10.1021/ja048113w.

DOI:10.1021/ja048113w

PMID:15225024

Abstract

We explore the adaptability at a protein-DNA interface using phage display to re-engineer a homeodomain to specifically recognize a chemically synthesized, unnatural nucleotide. The engineered homeodomain preferentially binds to the modified DNA with affinity and specificity similar to those of natural homeodomains.

摘要

我们利用噬菌体展示技术探索蛋白质-DNA界面的适应性，以重新设计一个同源结构域，使其能够特异性识别化学合成的非天然核苷酸。工程化的同源结构域优先与修饰后的DNA结合，其亲和力和特异性与天然同源结构域相似。