鞘脂C4羟基化影响酵母去污剂不溶性富含糖脂的膜的性质。

Sphingolipid C4 hydroxylation influences properties of yeast detergent-insoluble glycolipid-enriched membranes.

作者信息

Idkowiak-Baldys Jolanta, Grilley Michelle M, Takemoto Jon Y

机构信息

Department of Biology, Utah State University, Logan, UT 84322-5305, USA.

出版信息

FEBS Lett. 2004 Jul 2;569(1-3):272-6. doi: 10.1016/j.febslet.2004.05.074.

DOI:10.1016/j.febslet.2004.05.074

PMID:15225647

Abstract

Sphingoid base C4 hydroxylation is required for syringomycin E action on the yeast plasma membrane. Detergent-insoluble glycolipid-enriched membranes (DIGs) from a yeast strain lacking C4 hydroxylated sphingoid bases (sur2delta) are composed of linear membrane fragments instead of vesicular structures observed for wild-type DIGs, though they have similar lipid compositions and amounts of DIG marker proteins. Light-scattering bands collected from sur2delta after centrifugation of Triton X-100-treated cell lysates in continuous density gradients have lower buoyant densities than that of the wild-type. The results show that C4 hydroxylation influences the physical and structural properties of DIGs and suggest that syringomycin E interacts with lipid rafts.

摘要

鞘氨醇碱基C4羟基化是丁香霉素E作用于酵母质膜所必需的。来自缺乏C4羟基化鞘氨醇碱基的酵母菌株（sur2δ）的去污剂不溶性富含糖脂的膜（DIGs）由线性膜片段组成，而不是野生型DIGs中观察到的囊泡结构，尽管它们具有相似的脂质组成和DIG标记蛋白的量。在连续密度梯度中对经Triton X-100处理的细胞裂解物进行离心后，从sur2δ收集的光散射带的浮力密度低于野生型。结果表明，C4羟基化影响DIGs的物理和结构特性，并表明丁香霉素E与脂筏相互作用。