[肾综合征出血热疫苗血清学监测]

[Serological surveillance on vaccine against hemorrhagic fever with renal syndrome].

作者信息

Gong Zhen-yu, Weng Jing-qing, Zhao Zhi-ya, Chen Yu-tu, Zhu A-gen, Fu Gui-ming, Fang Chun-fu, Zhu Zhi-yong, Chen En-fu, Wang Chui-zhang, Wang Wei, Lei Lin-hai, Wang Zhen

机构信息

Center for Disease Control and Prevention, Zhejiang Province, Hangzhou 310009, China.

出版信息

Zhonghua Liu Xing Bing Xue Za Zhi. 2004 May;25(5):400-2.

PMID:15231163

Abstract

OBJECTIVE

To observe the serological and epidemiological efficacy of hemorrhagic fever renal syndrome (HFRS) vaccine in Zhejiang province.

METHODS

Immunofluorescent antibody assay and Mcro-CPE method were used to test specific IgG antibody and the titer of neutralizing antibody.

RESULTS

Two weeks after the injection of the third dose, the sero-conversion rates by both immunofluorescent antibody test (IgG) and neutralization test were 100.0% (67/67) (95% CI: 96.3 - 100.0) and 44.4% (8/18)(95% CI: 22.0 - 69.0) with geometric mean titers (GMTs) 72.1 and 4.6 respectively. The rates of seroconversion of immunofluorescent antibody by immunofluorescence antibody assay (IFA) were 28.6%, 83.3%, 75.0%, 53.1%, 22.6%, 10.0% and 55.0% before reinforcement, two weeks, one year, one year and a half years, two years, three years and five years after reinforcement. The rates of neutralizing antibody seroconversion by the Mcro-CPE method were found as 14.8%, 55.6%, 35.0%, 31.3%, 26.0%, 10.0% and 50.0% respectively. We found some antibody dependent immunization enhancement phenomenon among the inoculated population, but further observation was needed.

CONCLUSION

HFRS vaccine was immunologically effective and the duration of serous antibody last long.

摘要

目的

观察浙江省肾综合征出血热（HFRS）疫苗的血清学及流行病学效果。

方法

采用免疫荧光抗体法和微量CPE法检测特异性IgG抗体及中和抗体滴度。

结果

接种第三剂疫苗两周后，免疫荧光抗体试验（IgG）和中和试验的血清转化率分别为100.0%（67/67）（95%可信区间：96.3 - 100.0）和44.4%（8/18）（95%可信区间：22.0 - 69.0），几何平均滴度（GMTs）分别为72.1和4.6。加强免疫前、加强免疫后两周、一年、一年半、两年、三年和五年，免疫荧光抗体法检测的免疫荧光抗体血清转化率分别为28.6%、83.3%、75.0%、53.1%、22.6%、10.0%和55.0%。微量CPE法检测的中和抗体血清转化率分别为14.8%、55.6%、35.0%、31.3%、26.0%、1…显示全部