Manseth Even, Skjervold Per O, Flengsrud Ragnar
Department of Chemistry, Biotechnology and Food Science, Agricultural University of Norway, Chr.M.Falsensgate 1, P.O. Box 5040, N-1432 As, Norway.
J Biochem Biophys Methods. 2004 Jul 30;60(1):39-47. doi: 10.1016/j.jbbm.2004.04.016.
A modified method of sample displacement chromatography (SDC) was used to purify active salmon thrombin on a heparin-coupled matrix to near homogeneity in milligram amounts from 117 ml plasma. This was achieved by combining a low-pressure multi-column affinity chromatography system with non-homogenous sample application in the order of increasing affinity to Heparin Sepharose. The results suggest that this modified method could be useful in protein purification. Some characteristics of salmon thrombin are presented.
采用改良的样品置换色谱法（SDC），在肝素偶联基质上从117毫升血浆中纯化出毫克量的活性鲑鱼凝血酶，使其纯度接近均一。这是通过将低压多柱亲和色谱系统与以对肝素琼脂糖亲和力递增顺序进行的非均一样品应用相结合来实现的。结果表明，这种改良方法可能对蛋白质纯化有用。本文还介绍了鲑鱼凝血酶的一些特性。
Zotero 插件