Mint1是一种与Munc-18相互作用的蛋白，在分泌胰岛素的β细胞中表达。
Mint1, a Munc-18-interacting protein, is expressed in insulin-secreting beta-cells.
作者信息
Zhang Wei, Lilja Lena, Bark Christina, Berggren Per-Olof, Meister Björn
机构信息
Department of Neuroscience, Karolinska Institutet, SE-171 77, Stockholm, Sweden.
出版信息
Biochem Biophys Res Commun. 2004 Jul 30;320(3):717-21. doi: 10.1016/j.bbrc.2004.05.208.
Munc-18-interacting (Mint) proteins are adaptors involved in regulation of synaptic vesicle exocytosis. We have investigated expression and cellular localization of Mint1 in pancreatic islets with special reference to insulin-secreting beta-cells. Western blotting showed that Mint1 was expressed in hamster (HIT-T15) and rat (RINm5F) beta-cell lines. Mint1 immunoreactivity was preferentially localized to the periphery of individual islet cells. RT-PCR analysis revealed that apart from Mint1, RINm5F cells and rat islets also transcribed the mRNAs for Mint2 and Mint3. Expression of Mint proteins in pancreatic beta-cells suggests a functional role for these proteins in insulin granule exocytosis.
与Munc-18相互作用（Mint）蛋白是参与调节突触小泡胞吐作用的衔接蛋白。我们研究了Mint1在胰岛中的表达及细胞定位，特别关注分泌胰岛素的β细胞。蛋白质免疫印迹法显示，Mint1在仓鼠（HIT-T15）和大鼠（RINm5F）β细胞系中表达。Mint1免疫反应性优先定位于单个胰岛细胞的周边。逆转录聚合酶链反应（RT-PCR）分析显示，除Mint1外，RINm5F细胞和大鼠胰岛还转录Mint2和Mint3的信使核糖核酸（mRNA）。Mint蛋白在胰腺β细胞中的表达表明这些蛋白在胰岛素颗粒胞吐作用中具有功能作用。
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