通过电化学发光分析评估从各种过滤盒中回收微小隐孢子虫卵囊的效率。

Evaluation of Cryptosporidium parvum oocyst recovery efficiencies from various filtration cartridges by electrochemiluminescence assays.

作者信息

Lee Y, Gomez L L, McAuliffe I T, Tsang V C W

机构信息

Immunology Branch, Division of Parasitic Diseases, National Center for Infectious Disease, Centers for Disease Control and Prevention, Public Health Services, U.S. Department of Health and Human Services, Atlanta, GA 30341, USA.

出版信息

Lett Appl Microbiol. 2004;39(2):156-62. doi: 10.1111/j.1472-765X.2004.01552.x.

DOI:10.1111/j.1472-765X.2004.01552.x

PMID:15242454

Abstract

AIMS

To evaluate four types of filtration cartridges for their capacities, efficiency for capture and release of Cryptosporidium parvum oocysts for detection.

METHODS AND RESULTS

Filtration cartridges included in this evaluation were IDEXX Filta-Max, Gelman Envirochek HV, Corning CrypTest, and Filterite Sigma+. Various dosages of C. parvum oocysts were spiked into water samples with a wide range of turbidity (10-50 NTU). Electrochemiluminescence assays were employed to enumerate viable or total number of C. parvum oocysts in these eluates. Among the cartridges tested, Filta-Max consistently showed higher oocyst recovery efficiency, especially with large volume, highly turbid water samples.

CONCLUSIONS

Filta-Max filter is the best performer because of its higher oocyst recovery efficiency.

SIGNIFICANCE AND IMPACT OF THE STUDY

The overall sensitivities of various C. parvum oocyst detection assays in water samples can be improved if highly efficient oocyst recovery filtration cartridges such as Filta-Max are incorporated in sample preparation.

摘要

目的

评估四种过滤滤芯对微小隐孢子虫卵囊的捕获和释放能力及检测效率。

方法与结果

本评估中使用的过滤滤芯包括IDEXX Filta-Max、Gelman Envirochek HV、康宁Cryptest和Filterite Sigma+。将不同剂量的微小隐孢子虫卵囊添加到具有广泛浊度（10 - 50 NTU）的水样中。采用电化学发光分析法对这些洗脱液中活的或总的微小隐孢子虫卵囊进行计数。在所测试的滤芯中，Filta-Max始终显示出较高的卵囊回收效率，尤其是对于大体积、高浊度的水样。