从60日龄牛全胎儿和胎儿胎盘生成了总共6483个表达序列标签。

Generation of a total of 6483 expressed sequence tags from 60 day-old bovine whole fetus and fetal placenta.

作者信息

Oishi M, Gohma H, Lejukole H Y, Taniguchi Y, Yamada T, Suzuki K, Shinkai H, Uenishi H, Yasue H, Sasaki Y

机构信息

Laboratory of Animal Breeding and Genetics, Graduate School of Agriculture, Kyoto University, Sakyoku, Kyoto, Japan.

出版信息

Anim Biotechnol. 2004 May;15(1):1-8. doi: 10.1081/ABIO-120027479.

DOI:10.1081/ABIO-120027479

PMID:15248596

Abstract

Expressed sequence tags (ESTs) generated based on characterization of clones isolated randomly from cDNA libraries are used to study gene expression profiles in specific tissues and to provide useful information for characterizing tissue physiology. In this study, two directionally cloned cDNA libraries were constructed from 60 day-old bovine whole fetus and fetal placenta. We have characterized 5357 and 1126 clones, and then identified 3464 and 795 unique sequences for the fetus and placenta cDNA libraries: 1851 and 504 showed homology to already identified genes, and 1613 and 291 showed no significant matches to any of the sequences in DNA databases, respectively. Further, we found 94 unique sequences overlapping in both the fetus and the placenta, leading to a catalog of 4165 genes expressed in 60 day-old fetus and placenta. The catalog is used to examine expression profile of genes in 60 day-old bovine fetus and placenta.

摘要

基于从cDNA文库中随机分离的克隆的特征而产生的表达序列标签（ESTs），被用于研究特定组织中的基因表达谱，并为表征组织生理学提供有用信息。在本研究中，从60日龄的牛全胎和胎盘构建了两个定向克隆的cDNA文库。我们对5357个和1126个克隆进行了表征，然后分别为胎儿和胎盘cDNA文库鉴定出3464个和795个独特序列：1851个和504个与已鉴定的基因具有同源性，1613个和291个分别与DNA数据库中的任何序列均无显著匹配。此外，我们发现94个独特序列在胎儿和胎盘中都有重叠，从而形成了一个在60日龄胎儿和胎盘中表达的4165个基因的目录。该目录用于检查60日龄牛胎儿和胎盘基因的表达谱。