在含有[Os(bpy)₂(PVP)₁₀]²⁺的超薄膜中对氧化DNA进行直接电化学发光检测。
Direct electrochemiluminescence detection of oxidized DNA in ultrathin films containing [Os(bpy)2(PVP)10]2+.
作者信息
Dennany Lynn, Forster Robert J, White Blanaid, Smyth Malcolm, Rusling James F
机构信息
National Centre for Sensor Research, School of Chemical Sciences, Dublin City University, Dublin 9, Ireland.
出版信息
J Am Chem Soc. 2004 Jul 21;126(28):8835-41. doi: 10.1021/ja048615+.
Direct electrochemiluminescence (ECL) involving oxidized DNA was demonstrated in ultrathin films of cationic polymer [Os(bpy)2(PVP)10]2+ [PVP = poly(vinyl pyridine)] assembled layer-by-layer with DNA or oligonucleotides. Electrochemically oxidized Os(II) sites generated ECL from films containing oxo-guanines on DNA formed by chemical oxidation using Fenton reagent. Films combining DNA, [Ru(bpy)2(PVP)10]2+, and [Os(bpy)2(PVP)10]2+ had Os(II) sites that produced ECL specific for oxidized DNA, and Ru(II) sites gave ECL from reaction with oxo-adenines, chemically damaged DNA, and possibly from cleaved DNA strands.
在与DNA或寡核苷酸逐层组装的阳离子聚合物[Os(bpy)₂(PVP)₁₀]²⁺[PVP = 聚(乙烯基吡啶)]的超薄膜中，证明了涉及氧化DNA的直接电化学发光(ECL)。电化学氧化的Os(II)位点从含有通过使用芬顿试剂化学氧化形成的DNA上的氧代鸟嘌呤的薄膜中产生ECL。结合了DNA、[Ru(bpy)₂(PVP)₁₀]²⁺和[Os(bpy)₂(PVP)₁₀]²⁺的薄膜具有产生对氧化DNA特异的ECL的Os(II)位点，并且Ru(II)位点通过与氧代腺嘌呤、化学损伤的DNA反应以及可能与切割的DNA链反应产生ECL。
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