二氢叶酸还原酶的过量产生导致酵母对磺胺二氢蝶酸产生抗性。
Over-production of dihydrofolate reductase leads to sulfa-dihydropteroate resistance in yeast.
作者信息
Patel Onisha, Karnik Kuldeep, Macreadie Ian G
机构信息
CSIRO Health Sciences and Nutrition, Parkville, Vic., Australia.
出版信息
FEMS Microbiol Lett. 2004 Jul 15;236(2):301-5. doi: 10.1016/j.femsle.2004.06.001.
Dihydropteroate synthase (DHPS) can metabolise sulfa drugs into sulfa-dihydropteroate (sulfa-DHP), which inhibits cell growth through competition with dihydrofolate (DHF), possibly indicating dihydrofolate reductase (DHFR) as the target of sulfa-DHP. The effect of over-production of DHFR on sulfa-DHP resistance was examined in Saccharomyces cerevisiae using a strain that requires DHF for growth. This strain was transformed with a plasmid which encodes over-production of DHFR in the presence of CuSO4. Over-production led to resistance to sulfa-DHP suggesting that sulfa-DHP targets DHFR. Spontaneous mutants hyper-resistant to sulfa-DHP did not show any changes within DHFR.
二氢蝶酸合酶（DHPS）可将磺胺类药物代谢为磺胺二氢蝶酸（sulfa-DHP），后者通过与二氢叶酸（DHF）竞争来抑制细胞生长，这可能表明二氢叶酸还原酶（DHFR）是sulfa-DHP的作用靶点。使用一株生长需要DHF的酿酒酵母菌株，研究了DHFR过量表达对sulfa-DHP抗性的影响。该菌株用一种在硫酸铜存在下编码DHFR过量表达的质粒进行转化。过量表达导致对sulfa-DHP产生抗性，表明sulfa-DHP的作用靶点是DHFR。对sulfa-DHP具有高度抗性的自发突变体在DHFR内未显示任何变化。
Zotero 插件