琥珀酸沃林氏菌多硫化物还原酶活性位点的结构和功能类似物。

Structural and functional analogue of the active site of polysulfide reductase from Wolinella succinogenes.

作者信息

Nagarajan Kowliki, Joshi Hemant K, Chaudhury Pradeep K, Pal Kuntal, Cooney J Jon A, Enemark John H, Sarkar Sabyasachi

机构信息

Department of Chemistry, Indian Institute of Technology, Kanpur-208 016, India.

出版信息

Inorg Chem. 2004 Jul 26;43(15):4532-3. doi: 10.1021/ic049665i.

DOI:10.1021/ic049665i

PMID:15257571

Abstract

Synthesis of [PPh4]2[Mo(SPh)2(S2C2(CN)2)2] (2) from [PPh4]2[MoO(S2C2(CN)2)2] (1) has been achieved to mimic the postulated [Mo(S)6] core of polysulfide reductase with two thiolates and two bis(ene-dithiolate) ligands. Compound 2 reacts with polysulfide to yield H2S, modeling the function of polysulfide reductase. The facile conversion of 2 back to 1 in moist solvent suggests that the interconversion of the [MoIV = O] and [MoIV - X] (X = O-Ser, S-Cys, Se-Cys) moieties might occur in the DMSO reductase class of enzymes under appropriate hydrophobic/hydrophilic conditions.

摘要

已实现由[四苯基鏻]₂[MoO(S₂C₂(CN)₂)₂]（1）合成[四苯基鏻]₂[Mo(SPh)₂(S₂C₂(CN)₂)₂]（2），以模拟多硫化物还原酶假定的[Mo(S)₆]核心，该核心带有两个硫醇盐配体和两个双（烯二硫醇盐）配体。化合物2与多硫化物反应生成H₂S，模拟了多硫化物还原酶的功能。在潮湿溶剂中2能轻松转化回1，这表明在适当的疏水/亲水条件下，[MoIV = O]和[MoIV - X]（X = O - 丝氨酸、S - 半胱氨酸、Se - 半胱氨酸）部分在二甲基亚砜还原酶类酶中可能会发生相互转化。