TREK-1（一种双孔结构域钾离子通道）在小鼠和大鼠外周前庭系统中的定位。

Localization of TREK-1, a two-pore-domain K+ channel in the peripheral vestibular system of mouse and rat.

作者信息

Nicolas Marie-Thérèse, Lesage Florian, Reyes Roberto, Barhanin Jacques, Demêmes Danielle

机构信息

Université Montpellier 2, Place Eugène Bataillon, P.O. Box 089, 34095 Montpellier, cedex 05, France.

出版信息

Brain Res. 2004 Aug 13;1017(1-2):46-52. doi: 10.1016/j.brainres.2004.05.012.

DOI:10.1016/j.brainres.2004.05.012

PMID:15261098

Abstract

The distribution of two-pore-domain (2P-domain) K(+) channels of the TREK subfamily was studied using immunocytochemistry in the peripheral vestibular system of mouse and rat. Using RT-PCR, the mRNA for TREK-1, but not for TREK-2 or TRAAK, were detected in mouse vestibular endorgans and ganglia. The TREK-1 channel protein was immunodetected in both nerve fibers and nerve cell bodies in the vestibular ganglion, both afferent fibers and nerve calyces innervating type I hair cells in the utricle and cristae. The post-synaptic localization in afferent calyces may suggest a neuroprotective role in glutamatergic excitotoxicity during ischemic conditions. In non-neuronal cells, TREK-1 was immunodetected in the apical membrane of dark cells and transitional cells, both of which are involved in endolymph K(+) secretion and recycling. TREK-1 may subserve some neuroprotective function in afferent nerve fibers as well as play a role in endolymph potassium homeostasis.

摘要

利用免疫细胞化学方法，在小鼠和大鼠的外周前庭系统中研究了TREK亚家族双孔结构域（2P结构域）钾通道的分布。通过逆转录聚合酶链反应（RT-PCR），在小鼠前庭终器和神经节中检测到了TREK-1的信使核糖核酸（mRNA），但未检测到TREK-2或TRAAK的mRNA。在前庭神经节的神经纤维和神经细胞体中，以及在椭圆囊和嵴中支配I型毛细胞的传入纤维和神经壶腹内，均免疫检测到了TREK-1通道蛋白。传入壶腹的突触后定位可能提示其在缺血状态下对谷氨酸能兴奋性毒性具有神经保护作用。在非神经元细胞中，在暗细胞和过渡细胞的顶端膜中免疫检测到了TREK-1，这两种细胞均参与内淋巴钾离子的分泌和再循环。TREK-1可能在传入神经纤维中发挥某种神经保护功能，并在内淋巴钾离子稳态中起作用。