四膜虫中表皮生长因子（EGF）和表皮生长因子受体样免疫反应性的存在与定位。

Presence and localization of epidermal growth factor (EGF)- and EGF-receptor-like immunoreactivity in Tetrahymena.

作者信息

Csaba G, Kovács P, Pállinger Eva

机构信息

Department of Genetics, Cell and Immunobiology, Semmelweis University and Molecular Immunological Research Group of the Hungarian Academy of Sciences, Budapest, Hungary.

出版信息

Cell Biol Int. 2004;28(7):491-6. doi: 10.1016/j.cellbi.2004.04.002.

DOI:10.1016/j.cellbi.2004.04.002

PMID:15261155

Abstract

The presence of EGF- and EGF-receptor-like immunoreactivity in Tetrahymena was studied with the help of FITC-labelled monoclonal antibodies, using flow cytometry and confocal microscopy. Tetrahymena has endogeneous EGF and treatment with it significantly increases the hormone content of the cells. The hormone is diffusely localized, particularly in the cytopharynx, where it forms a structure larger than 10 microm. EGF-receptors are also demonstrable, particularly in the cortical region in connection with cilia, and EGF treatment significantly enhances cortical fluorescence. The relationships between these observations and literary data on the effects of EGF in Tetrahymena are discussed.

摘要

借助异硫氰酸荧光素标记的单克隆抗体，采用流式细胞术和共聚焦显微镜技术，研究了四膜虫中表皮生长因子（EGF）和表皮生长因子受体样免疫反应性的存在情况。四膜虫具有内源性表皮生长因子，用其处理可显著增加细胞中的激素含量。该激素呈弥漫性定位，尤其在胞咽中，在那里它形成一个大于10微米的结构。表皮生长因子受体也可被检测到，特别是在与纤毛相关的皮质区域，表皮生长因子处理可显著增强皮质荧光。讨论了这些观察结果与关于表皮生长因子对四膜虫作用的文献数据之间的关系。