使用交联剂分离小龙虾（奥氏螯虾）光感受器的横纹肌微绒毛细胞骨架。

Isolation of the rhabdomeral microvillar cytoskeleton of the crayfish (Orconectes limosus) photoreceptor by a crosslinking reagent.

Schraermeyer U, Rack M, Stieve H

Institut für Biologie II (Zoologie), RWTH Aachen, Germany.

Comp Biochem Physiol B. 1992 May;102(1):43-8. doi: 10.1016/0305-0491(92)90270-2.

Abstract

Rhabdomeral microvilli of photoreceptor cells of invertebrates contain a labile central cytoskeleton. For stabilization of the rhabdomeral cytoskeleton of the crayfish Orconectes limosus the crosslinking reagent suberic acid bis (N-hydroxysuccinimide ester) was used. 2. It was found that this crosslinking reagent can be successfully used to stabilize and isolate the microvillar cytoskeleton of crayfish photoreceptors. 3. After detergent treatment cytosolic proteins and the cell membranes were removed. 4. By the combined use of crosslinker and detergent the accessibility of antibodies or other markers to the microvillar cytoskeleton is possible. 5. This method may be useful, because at present little is known about the proteins associated with the central filament of invertebrate photoreceptors.

摘要

无脊椎动物感光细胞的横纹肌微绒毛含有不稳定的中央细胞骨架。为了稳定小龙虾奥氏螯虾横纹肌的细胞骨架，使用了交联剂辛二酸双（N-羟基琥珀酰亚胺酯）。2. 发现这种交联剂可成功用于稳定和分离小龙虾感光细胞的微绒毛细胞骨架。3. 经去污剂处理后，去除了胞质蛋白和细胞膜。4. 通过联合使用交联剂和去污剂，抗体或其他标记物有可能作用于微绒毛细胞骨架。5. 这种方法可能有用，因为目前对于与无脊椎动物感光细胞中央细丝相关的蛋白质了解甚少。