利用嗜热栖热放线菌属菌株TP6-B1的β-半乳糖苷酶，酶催化合成半乳糖基化的1D-和1L-手性肌醇、1D-松醇、肌醇及选定的衍生物。

Enzyme-catalysed synthesis of galactosylated 1D- and 1L-chiro-inositol, 1D-pinitol, myo-inositol and selected derivatives using the beta-galactosidase from the thermophile Thermoanaerobacter sp. strain TP6-B1.

作者信息

Hart Joanne B, Kröger Lars, Falshaw Andrew, Falshaw Ruth, Farkas Erzsébet, Thiem Joachim, Win Anna L

机构信息

Carbohydrate Chemistry Team, Industrial Research Ltd, PO Box 31-310, Lower Hutt, New Zealand.

出版信息

Carbohydr Res. 2004 Aug 2;339(11):1857-71. doi: 10.1016/j.carres.2004.05.016.

DOI:10.1016/j.carres.2004.05.016

PMID:15261579

Abstract

The products from the enzymatic beta-D-galactopyranosylation of 1D-chiro-inositol, 1D-pinitol, 1D-3-O-allyl-4-O-methyl-chiro-inositol, 1D-3,4-di-O-methyl-chiro-inositol, 1L-chiro-inositol and myo-inositol in combined yields ranging from 46% to 64% have been obtained using the beta-galactosidase isolated from an anaerobic extreme thermophile, Thermoanaerobacter sp. strain TP6-B1 and p-nitrophenyl beta-D-galactopyranoside as the donor. Analysis of the products from these reactions reveals information about the acceptor preferences of the enzyme.

摘要

使用从厌氧极端嗜热菌嗜热厌氧杆菌属菌株TP6 - B1中分离出的β - 半乳糖苷酶和对硝基苯基β - D - 吡喃半乳糖苷作为供体，已获得1D - 手性肌醇、1D - 松醇、1D - 3 - O - 烯丙基 - 4 - O - 甲基 - 手性肌醇、1D - 3,4 - 二 - O - 甲基 - 手性肌醇、1L - 手性肌醇和肌醇的酶促β - D - 吡喃半乳糖基化产物，总产率在46%至64%之间。对这些反应产物的分析揭示了该酶的受体偏好信息。