墨西哥女性散发性乳腺癌中比较基因组杂交技术对染色体变化的鉴定

Identification of chromosomal changes with comparative genomic hybridization in sporadic breast cancer in Mexican women.

作者信息

Valladares Adán, Salamanca Fabio, Madrigal-Bujaidar Eduardo, Arenas Diego

机构信息

Laboratorio de Genética Molecular, Unidad de Investigación Médica en Genética Humana, Centro Médico Nacional Siglo XXI, IMSS, Av. Cuauhtémoc 330, Col. Doctores, 06725 Mexico City, D.F., Mexico.

出版信息

Cancer Genet Cytogenet. 2004 Jul 15;152(2):163-6. doi: 10.1016/j.cancergencyto.2003.11.016.

DOI:10.1016/j.cancergencyto.2003.11.016

PMID:15262439

Abstract

Breast cancer is the second leading cause of death in women older than 35 years in Mexico. In this study, we used comparative genomic hybridization (CGH) to analyze sporadic breast cancers at stages II and III from untreated patients. We obtained 4.1 chromosomal alterations per sample, less than in previous reports. We identified a small region in Xq27 with high-level amplification in 3 of 16 samples. This amplification has been reported only in pancreatic and gastric cancer cell lines and in testis tumors; in addition, this amplification had been reported in one primary breast cancer, but in a more extended region that we identified. We also identified a loss in 2p13, not previously reported in this neoplasia. The most frequent alterations were amplifications in 4q, 5q, 8q, 12p, and 13q and losses in 1p, 8p, 16p, 19q, and Xp. CGH provides data for better understanding of molecular events in this neoplasia.

摘要

在墨西哥，乳腺癌是35岁以上女性的第二大死因。在本研究中，我们使用比较基因组杂交（CGH）技术分析未经治疗的II期和III期散发性乳腺癌。我们每个样本获得4.1个染色体改变，少于先前报告。我们在16个样本中的3个样本中发现Xq27有一个小区域存在高水平扩增。这种扩增仅在胰腺和胃癌细胞系以及睾丸肿瘤中报道过；此外，这种扩增在一例原发性乳腺癌中也有报道，但在我们确定的区域更广泛。我们还发现2p13存在缺失，此前在该肿瘤中未报道过。最常见的改变是4q、5q、8q、12p和13q的扩增以及1p、8p、16p、19q和Xp的缺失。CGH为更好地理解该肿瘤中的分子事件提供了数据。