以甲基对硫磷水解酶基因mpd为报告基因的启动子捕获载体的构建与应用。
Construction and application of a promoter-trapping vector with methyl parathion hydrolase gene mpd as the reporter.
作者信息
Cui Zhong-Li, Zhang Xiao-Zhou, Zhang Zhong-Hui, Li Shun-Peng
机构信息
Department of Microbiology, MOA Key Laboratory of Microbiological Engineering of the Agricultural Environment, Nanjing Agricultural University, Nanjing 210095, P.R. China.
出版信息
Biotechnol Lett. 2004 Jul;26(14):1115-8. doi: 10.1023/B:BILE.0000035481.03854.41.
A facilitative and efficient promoter-trapping vector, pUC-mpd, was constructed with the promoterless methyl parathion hydrolase gene as the reporter. This reporter gene is easily used to clone promoters with different promoting strength on selective plates. Promoter regions of the ytkA and ywoF genes with strong promoting and signal peptide functions were cloned from the Bacillus subtilis 168 genomic promoter library with this vector.
构建了一种促进性且高效的启动子捕获载体pUC-mpd，以无启动子的甲基对硫磷水解酶基因作为报告基因。该报告基因易于用于在选择性平板上克隆具有不同促进强度的启动子。利用此载体从枯草芽孢杆菌168基因组启动子文库中克隆了具有强促进和信号肽功能的ytkA和ywoF基因的启动子区域。
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