Low dose insemination in the mare: an update.

The generally recommended minimum number of spermatozoa required for conventional artificial insemination in the mare is in excess of 200 x 10(6) progressively motile spermatozoa. Recent developments in different insemination techniques such as deep uterine, hysteroscopic and oviductal insemination, which have been designed to use significantly fewer spermatozoa, are reviewed in this paper. A number of studies have demonstrated that ultrasound guided deep uterine insemination of 5 x 10(6) fresh spermatozoa can produce satisfactory pregnancy rates. The use of hysteroscopic insemination enables the insemination dose to be successfully reduced to 1 x 10(6) fresh or 3 x 10(6) frozen-thawed spermatozoa. Further reduction of the insemination dose is possible and satisfactory pregnancy rates can also be achieved by surgical oviductal insemination of mares with as few as 2 x 10(5) fresh spermatozoa. The refinement of these insemination techniques will allow us to maximise the use of frozen-thawed semen, use new technology such as sex-preselection of spermatozoa and to conserve and utilise epididymal spermatozoa at the time of castration or death of a stallion.