An Y-J, Joo Y-H, Hong I-Y, Ryu H-W, Cho K-S
Department of Environmental Science, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, 143-701, Seoul, Korea.
Appl Microbiol Biotechnol. 2004 Oct;65(5):611-9. doi: 10.1007/s00253-004-1596-4. Epub 2004 Jul 20.
The degradation characteristics of toluene coupled to nitrate reduction were investigated in enrichment culture and the microbial communities of toluene-degrading denitrifying consortia were characterized by denaturing gradient gel electrophoresis (DGGE) technique. Anaerobic nitrate-reducing bacteria were enriched from oil-contaminated soil samples collected from terrestrial (rice field) and marine (tidal flat) ecosystems. Enriched consortia degraded toluene in the presence of nitrate as a terminal electron acceptor. The degradation rate of toluene was affected by the initial substrate concentration and co-existence of other hydrocarbons. The types of toluene-degrading denitrifying consortia depended on the type of ecosystem. The clone RS-7 obtained from the enriched consortium of the rice field was most closely related to a toluene-degrading and denitrifying bacterium, Azoarcus denitrificians (A. tolulyticus sp. nov.). The clone TS-11 detected in the tidal flat enriched consortium was affiliated to Thauera sp. strain S2 (T. aminoaromatica sp. nov.) that was able to degrade toluene under denitrifying conditions. This indicates that environmental factors greatly influence microbial communities obtained from terrestrial (rice field) and marine (tidal flat) ecosystems.
在富集培养中研究了甲苯与硝酸盐还原耦合的降解特性，并通过变性梯度凝胶电泳（DGGE）技术对甲苯降解反硝化菌群的微生物群落进行了表征。从陆地（稻田）和海洋（潮滩）生态系统采集的石油污染土壤样本中富集厌氧硝酸盐还原菌。富集的菌群在以硝酸盐作为末端电子受体的情况下降解甲苯。甲苯的降解速率受初始底物浓度和其他烃类共存的影响。甲苯降解反硝化菌群的类型取决于生态系统类型。从稻田富集菌群中获得的克隆RS-7与甲苯降解反硝化细菌反硝化偶氮弧菌（新种甲苯分解偶氮弧菌）关系最为密切。在潮滩富集菌群中检测到的克隆TS-11隶属于陶厄氏菌属菌株S2（新种氨基芳香陶厄氏菌），该菌株能够在反硝化条件下降解甲苯。这表明环境因素极大地影响了从陆地（稻田）和海洋（潮滩）生态系统获得的微生物群落。
